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HAMAP rule MF_00126

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General rule information [?]

Accession MF_00126
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 31)
Name Gln_tRNA_synth
Scope(s) Bacteria
Template(s) P00962 (SYQ_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00126 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SYQ
Protein name RecName: Full=Glutamine--tRNA ligase;
                 EC=6.1.1.18;
AltName: Full=Glutaminyl-tRNA synthetase;
                 Short=GlnRS;
Gene name Name=glnS;

Comments [?]

CATALYTIC ACTIVITY Reaction=ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L- glutaminyl-tRNA(Gln); Xref=Rhea:RHEA:20121, Rhea:RHEA-COMP:9662, Rhea:RHEA-COMP:9681, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58359, ChEBI:CHEBI:78442, ChEBI:CHEBI:78521, ChEBI:CHEBI:456215; EC=6.1.1.18;
SUBUNITMonomer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the class-I aminoacyl-tRNA synthetase family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004819; Molecular function:glutamine-tRNA ligase activity
GO:0006424; Biological process:glutamyl-tRNA aminoacylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00749; tRNA-synt_1c; 1;
PRINTS PR00987; TRNASYNTHGLU; 1;
NCBIfam TIGR00440; glnS; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;

Features [?]

From: SYQ_ECOLI (P00962)
Key From To Description Tag Condition FTGroup
BINDING 35 37 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
[ED]-P-N
BINDING 41 47 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
H-[IL]-G-H-[AS]-K-[SA]
BINDING 261 262 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
R-L
BINDING 269 271 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
[MVL]-S-K
REGION 317 324 /note="Interaction with tRNA" T-K-Q-D-N-T-I-E
MOTIF 34 44 /note="'HIGH' region" P-[ED]-P-[NS]-G-[YF]-x-H-[LI]-G-H
MOTIF 268 272 /note="'KMSKS' region" [LVI]-[LMT]-S-K-R
BINDING 67 67 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
D
BINDING 212 212 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
Y
BINDING 231 231 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
T

Additional information [?]

Size range 552-636 amino acids
Related rules None
Fusion Nter: None Cter: MF_00121 (gatB)
Comments Partial gatB like C-terminal domain in DEIRA. Possible wrong start site in DEIRA.



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