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HAMAP rule MF_00140

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General rule information [?]

Accession MF_00140
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 41)
Name Trp_tRNA_synth
Scope(s) Bacteria
Archaea
Template(s) P00953 (SYW_GEOSE); P21656 (SYW_BACSU); P00954 (SYW_ECOLI); Q9RVD6 (SYW2_DEIRA); Q58810 (SYW_METJA); [ Recover all ]
Triggered by
case c? <OC:Bacteria>
HAMAP; MF_00140_B (Get profile general information and statistics)
end case
case c? <OC:Archaea>
HAMAP; MF_00140_A (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SYW
Protein name RecName: Full=Tryptophan--tRNA ligase;
                 EC=6.1.1.2;
AltName: Full=Tryptophanyl-tRNA synthetase;
                 Short=TrpRS;
Gene name Name=trpS;

Comments [?]

FUNCTIONCatalyzes the attachment of tryptophan to tRNA(Trp).
CATALYTIC ACTIVITY Reaction=ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L- tryptophanyl-tRNA(Trp); Xref=Rhea:RHEA:24080, Rhea:RHEA-COMP:9671, Rhea:RHEA-COMP:9705, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57912, ChEBI:CHEBI:78442, ChEBI:CHEBI:78535, ChEBI:CHEBI:456215; EC=6.1.1.2;
case <OC:Bacteria>
SUBUNITHomodimer.
end case
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the class-I aminoacyl-tRNA synthetase family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004830; Molecular function:tryptophan-tRNA ligase activity
GO:0006436; Biological process:tryptophanyl-tRNA aminoacylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00579; tRNA-synt_1b; 1;
PRINTS PR01039; TRNASYNTHTRP; 1;
NCBIfam TIGR00233; TrpS; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;

Features [?]

From: SYW_GEOSE (P00953)
Key From To Description Tag Condition FTGroup
case <OC:Bacteria>
BINDING 9 11 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
[QTRK]-[PATS]-x(0,1)-[ST]
BINDING 17 18 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
G-[NH]
BINDING 144 146 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
G-x-D
BINDING 192 196 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
K-M-[SG]-K-S
MOTIF 10 18 /note="'HIGH' region" [PATS]-x(0,1)-[STA]-[GDN]-x-[ILVFYQP]-[HST]-[ILW]-G-[NH]
MOTIF 192 196 /note="'KMSKS' region" K-M-[SG]-K-S
BINDING 132 132 /ligand="L-tryptophan"
/ligand_id="ChEBI:CHEBI:57912"
D
BINDING 183 183 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
[VIL]
end case
From: SYW_METJA (Q58810)
Key From To Description Tag Condition FTGroup
case <OC:Archaea>
MOTIF 75 83 /note="'HIGH' region" P-[ST]-x(2)-[MVFP]-H-[LIF]-G-[HN]
MOTIF 255 259 /note="'KMSKS' region" K-M-S-[SA]-[SN]
end case

Additional information [?]

Size range 364-437 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Weird insertions in AQUAE, RALSO and METMA; not shown in alignment. DEIRA has a second tryptophanyl-tRNA synthetase (DR1093), which shows a weaker activity and whose role in protein biosynthesis has not been proven. It is probably involved in other processes.



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