HAMAP rule MF_00144
General rule information
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| Accession | MF_00144 |
| Dates | 1-JUN-2001 (Created)
2-SEP-2024 (Last updated, Version 37) |
| Name | tRNA_thiouridyl_MnmA |
| Scope(s) |
Bacteria |
| Template(s) | P25745 (MNMA_ECOLI); Q97T38 (MNMA_STRPN); [ Recover all ] |
| Triggered by |
HAMAP; MF_00144 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MNMA |
| Protein name | RecName: Full=tRNA-specific 2-thiouridylase MnmA; EC=2.8.1.13; |
| Gene name | Name=mnmA; |
Comments
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| case <OC:Enterobacterales> | |
| FUNCTION | Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs. |
| SUBUNIT | Interacts with TusE. |
| else | |
| FUNCTION | Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=uridine(34) in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 2-thiouridine(34) in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H(+); Xref=Rhea:RHEA:47032, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:11726, Rhea:RHEA-COMP:11727, Rhea:RHEA-COMP:11728, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499, ChEBI:CHEBI:29950, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:61963, ChEBI:CHEBI:65315, ChEBI:CHEBI:87170, ChEBI:CHEBI:456215; EC=2.8.1.13; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the MnmA/TRMU family. |
Keywords
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Gene Ontology
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| GO:0016740; Molecular function:transferase activity |
| GO:0006400; Biological process:tRNA modification |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: MNMA_ECOLI (P25745) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 11 | 18 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[GAL]-x-S-G-x-x-[DE]-S | ||||||||
| REGION | 97 | 99 | /note="Interaction with target base in tRNA" | N-P-D | ||||||||
| REGION | 149 | 151 | /note="Interaction with tRNA" | [KR]-[DEN]-Q | ||||||||
| REGION | 311 | 312 | /note="Interaction with tRNA" | [RK]-Y | ||||||||
| ACT_SITE | 102 | 102 | /note="Nucleophile" | C | ||||||||
| ACT_SITE | 199 | 199 | /note="Cysteine persulfide intermediate" | C | ||||||||
| BINDING | 37 | 37 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[MLFI] | ||||||||
| BINDING | 127 | 127 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
| SITE | 128 | 128 | /note="Interaction with tRNA" | H | ||||||||
| SITE | 344 | 344 | /note="Interaction with tRNA" | Q | ||||||||
| DISULFID | 102 | 199 | /note="Alternate" | disulf | C-x*-C | |||||||
Additional information
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| Size range | 338-431 amino acids |
| Related rules |
None |
| Fusion | Nter: <nifU>; <ribF> Cter: None |
| Comments | Shorter N-terminus in THEM4 and in the second copy of SYNAS; sequences not included in alignment and not taken into account in size range. |