HAMAP

Accession	MF_00144
Dates	1-JUN-2001 (Created) 9-DEC-2011 (Last updated, Version 24)

Data class

Protein

Predictors

HAMAP; MF_00144; [distribution of match scores in UniProtKB];[seed alignment for MF_00144]

Names

tRNA_thiouridyl_MnmA

Identifier

MNMA

Protein name

RecName: Full=tRNA-specific 2-thiouridylase mnmA; EC=2.8.1.-;

Gene name

mnmA

FUNCTION: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by iscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs (By similarity).

SUBUNIT: Interacts with tusE (By similarity).

FUNCTION: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34 (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the mnmA/TRMU family.

Pfam	PF03054; tRNA_Me_trans; 1;
TIGRFAMs	TIGR00420; TrmU; 1;

ATP-binding, Cytoplasm, Nucleotide-binding, RNA-binding, Transferase, tRNA processing, tRNA-binding.

GO:0016740; Molecular function: transferase activity.
GO:0006400; Biological process: tRNA modification.
GO:0005737; Cellular component: cytoplasm.

Disulfide bond.

From: MNMA_ECOLI (P25745)
Key		From		To		Description		Condition		FTGroup
NP_BIND		11		18		ATP (By similarity)		[GAL]-x-S-G-x-x-[DE]-S
REGION (Optional)		97		99		Interaction with target base in tRNA (By similarity)		N-P-D
REGION (Optional)		149		151		Interaction with tRNA (By similarity)		[KR]-[DEN]-Q
REGION (Optional)		311		312		Interaction with tRNA (By similarity)		[RK]-Y
ACT_SITE		102		102		Nucleophile (By similarity)		C
ACT_SITE		199		199		Cysteine persulfide intermediate (By similarity)		C
BINDING (Optional)		37		37		ATP; via amide nitrogen and carbonyl oxygen (By similarity)		[MLFI]
BINDING (Optional)		127		127		ATP; via amide nitrogen (By similarity)		G
SITE (Optional)		128		128		Interaction with tRNA (By similarity)		H
SITE (Optional)		344		344		Interaction with tRNA (By similarity)		Q
DISULFID		102		199		Alternate (By similarity)		C-x*-C

Size range:	338-431 amino acids
Related UniRules:	None
Template:	P25745 (MNMA_ECOLI); Q97T38 (MNMA_STRPN): [Recover all]
Scope:	Bacteria
Fusion:	Nter: <nifU>, <ribF>; Cter: None
Duplicate:	in ARCB4, BACFN, BACFR, BACTN, BACV8, CLOB1, CLOBH, CLOBK, CLOBL, CLOBM, CLOTE, FUSNN, GEOKA, SYNAS, THEP3, THEPX, THETN, XANOP
Plasmid encoded:	None
Comments:	Shorter N-terminus in THEM4 and in the second copy of SYNAS; sequences not included in alignment and not taken into account in size range.