HAMAP rule MF_00144
General rule information
[?]
Accession | MF_00144 |
Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 36) |
Name | tRNA_thiouridyl_MnmA |
Scope(s) |
Bacteria |
Template(s) | P25745 (MNMA_ECOLI); Q97T38 (MNMA_STRPN); [ Recover all ] |
Triggered by |
HAMAP; MF_00144 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Identifier | MNMA |
Protein name | RecName: Full=tRNA-specific 2-thiouridylase MnmA; EC=2.8.1.13; |
Gene name | Name=mnmA; |
Comments
[?]
case <OC:Enterobacterales> | |
FUNCTION | Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs. |
SUBUNIT | Interacts with TusE. |
else | |
FUNCTION | Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. |
end case | |
CATALYTIC ACTIVITY | Reaction=AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L- cysteinyl-[protein]; Xref=Rhea:RHEA:47032, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:11726, Rhea:RHEA-COMP:11727, Rhea:RHEA-COMP:11728, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499, ChEBI:CHEBI:29950, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:61963, ChEBI:CHEBI:65315, ChEBI:CHEBI:87170, ChEBI:CHEBI:456215; EC=2.8.1.13; |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | Belongs to the MnmA/TRMU family. |
Keywords
[?]
Gene Ontology
[?]
GO:0016740; Molecular function:transferase activity |
GO:0006400; Biological process:tRNA modification |
GO:0005737; Cellular component:cytoplasm |
Cross-references
[?]
Features
[?]
From: MNMA_ECOLI (P25745) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 11 | 18 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[GAL]-x-S-G-x-x-[DE]-S | ||||||||
REGION | 97 | 99 | /note="Interaction with target base in tRNA" | N-P-D | ||||||||
REGION | 149 | 151 | /note="Interaction with tRNA" | [KR]-[DEN]-Q | ||||||||
REGION | 311 | 312 | /note="Interaction with tRNA" | [RK]-Y | ||||||||
ACT_SITE | 102 | 102 | /note="Nucleophile" | C | ||||||||
ACT_SITE | 199 | 199 | /note="Cysteine persulfide intermediate" | C | ||||||||
BINDING | 37 | 37 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[MLFI] | ||||||||
BINDING | 127 | 127 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
SITE | 128 | 128 | /note="Interaction with tRNA" | H | ||||||||
SITE | 344 | 344 | /note="Interaction with tRNA" | Q | ||||||||
DISULFID | 102 | 199 | /note="Alternate" | disulf | C-x*-C |
Additional information
[?]
Size range | 338-431 amino acids |
Related rules |
None |
Fusion | Nter: <nifU>; <ribF> Cter: None |
Comments | Shorter N-terminus in THEM4 and in the second copy of SYNAS; sequences not included in alignment and not taken into account in size range. |