HAMAP

Accession	MF_00152
Dates	1-JUN-2001 (Created) 16-MAY-2011 (Last updated, Version 24)

Data class

Protein

Predictors

HAMAP; MF_00152; [distribution of match scores in UniProtKB];[seed alignment for MF_00152]

Names

Nfo

Identifier

END4

Protein name

RecName: Full=Probable endonuclease 4; EC=3.1.21.2; AltName: Full=Endodeoxyribonuclease IV; AltName: Full=Endonuclease IV;

Gene name

nfo

FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin (By similarity).

CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.

COFACTOR: Binds 3 zinc ions (By similarity).

SIMILARITY: Belongs to the AP endonuclease 2 family.

Pfam	PF01261; AP_endonuc_2; 1;
TIGRFAMs	TIGR00587; Nfo; 1;
PROSITE	PS00729; AP_NUCLEASE_F2_1; 1; PS00730; AP_NUCLEASE_F2_2; 1; PS00731; AP_NUCLEASE_F2_3; 1; PS51432; AP_NUCLEASE_F2_4; 1;

Hydrolase, Nuclease, Endonuclease, DNA damage, DNA repair, Metal-binding, Zinc.

GO:0008833; Molecular function: deoxyribonuclease IV (phage-T4-induced) activity.
GO:0008270; Molecular function: zinc ion binding.
GO:0006281; Biological process: DNA repair.

From: END4_ECOLI (P0A6C1)
Key		From		To		Description		Condition		FTGroup
METAL		69		69		Zinc 1 (By similarity)		H
METAL		109		109		Zinc 1 (By similarity)		H
METAL		145		145		Zinc 1 (By similarity)		E
METAL		145		145		Zinc 2 (By similarity)		E
METAL		179		179		Zinc 2 (By similarity)		D
METAL		182		182		Zinc 3 (By similarity)		H
METAL		216		216		Zinc 2 (By similarity)		H
METAL		229		229		Zinc 3 (By similarity)		D
METAL		231		231		Zinc 3 (By similarity)		H
METAL		261		261		Zinc 2 (By similarity)		E

Size range:	252-310 amino acids
Related UniRules:	None
Template:	P0A6C1 (END4_ECOLI)
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None