Annotation rule MF_00152
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General rule information [?]

Accession MF_00152
Dates 1-JUN-2001 (Created)
16-MAY-2011 (Last updated, Version 24)
Name Nfo
Scope Bacteria
Archaea

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
END4
Protein name
RecName: Full=Probable endonuclease 4;
EC=3.1.21.2;
AltName: Full=Endodeoxyribonuclease IV;
AltName: Full=Endonuclease IV;
Gene name
nfo

Comments [?]

Function Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin (By similarity).
Catalytic activity Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.
Cofactor Binds 3 zinc ions (By similarity).
Similarity Belongs to the AP endonuclease 2 family.

Keywords [?]


Gene Ontology [?]

GO:0008833; Molecular function: deoxyribonuclease IV (phage-T4-induced) activity.
GO:0008270; Molecular function: zinc ion binding.
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam PF01261; AP_endonuc_2; 1;
TIGRFAMs TIGR00587; Nfo; 1;
PROSITE PS00729; AP_NUCLEASE_F2_1; 1;
PS00730; AP_NUCLEASE_F2_2; 1;
PS00731; AP_NUCLEASE_F2_3; 1;
PS51432; AP_NUCLEASE_F2_4; 1;

Features [?]

From: END4_ECOLI (P0A6C1)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     69     69       Zinc 1 (By similarity)     H  
METAL     109     109       Zinc 1 (By similarity)     H  
METAL     145     145       Zinc 1 (By similarity)     E  
METAL     145     145       Zinc 2 (By similarity)     E  
METAL     179     179       Zinc 2 (By similarity)     D  
METAL     182     182       Zinc 3 (By similarity)     H  
METAL     216     216       Zinc 2 (By similarity)     H  
METAL     229     229       Zinc 3 (By similarity)     D  
METAL     231     231       Zinc 3 (By similarity)     H  
METAL     261     261       Zinc 2 (By similarity)     E  

Additional information [?]

Size range 252-310 amino acids
Related rules None
Template P0A6C1 (END4_ECOLI)
Fusion None