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HAMAP rule MF_00156
General rule information
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| Accession | MF_00156 |
| Dates | 1-JUN-2001 (Created)
28-NOV-2023 (Last updated, Version 43) |
| Name | PanB |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P9WIL7 (PANB_MYCTU); P31057 (PANB_ECOLI); Q9X712 (PANB_CORGL); Q9JZW6 (PANB_NEIMB); [ Recover all ] |
| Triggered by |
HAMAP; MF_00156 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PANB |
| Protein name | RecName: Full=3-methyl-2-oxobutanoate hydroxymethyltransferase; EC=2.1.2.11; AltName: Full=Ketopantoate hydroxymethyltransferase; Short=KPHMT; |
| Gene name | Name=panB; |
Comments
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| FUNCTION | Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha- ketoisovalerate to form ketopantoate. |
| CATALYTIC ACTIVITY | Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2- oxobutanoate + H2O = (6S)-5,6,7,8-tetrahydrofolate + 2- dehydropantoate; Xref=Rhea:RHEA:11824, ChEBI:CHEBI:11561, ChEBI:CHEBI:11851, ChEBI:CHEBI:15377, ChEBI:CHEBI:15636, ChEBI:CHEBI:57453; EC=2.1.2.11; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| case <OC:Bacteria> | |
| PATHWAY | Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)- pantoate from 3-methyl-2-oxobutanoate: step 1/2. |
| else case <OC:Archaea> | |
| PATHWAY | Cofactor biosynthesis; coenzyme A biosynthesis. |
| end case | |
| SUBUNIT | Homodecamer; pentamer of dimers. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the PanB family. |
Keywords
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| Cytoplasm | |
| Magnesium | |
| Metal-binding | |
| case <OC:Bacteria> | |
| Pantothenate biosynthesis | |
| else case <OC:Archaea> | |
| Coenzyme A biosynthesis | |
| end case | |
| Transferase | |
Gene Ontology
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| GO:0003864; Molecular function:3-methyl-2-oxobutanoate hydroxymethyltransferase activity | |
| case <OCellular component:Bacteria> | |
| GO:0015940; Biological process:pantothenate biosynthetic process | |
| else case <OCellular component:Archaea> | |
| GO:0015937; Biological process:coenzyme A biosynthetic process | |
| end case | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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Features
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| From: PANB_ECOLI (P31057) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 45 | 46 | /ligand="3-methyl-2-oxobutanoate" /ligand_id="ChEBI:CHEBI:11851" |
|||||||||
| ACT_SITE | 181 | 181 | /note="Proton acceptor" | E | ||||||||
| BINDING | 45 | 45 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 84 | 84 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 114 | 114 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | ||||||||
| BINDING | 84 | 84 | /ligand="3-methyl-2-oxobutanoate" /ligand_id="ChEBI:CHEBI:11851" |
D | ||||||||
| BINDING | 112 | 112 | /ligand="3-methyl-2-oxobutanoate" /ligand_id="ChEBI:CHEBI:11851" |
K | ||||||||
Additional information
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| Size range | 257-326 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Most archaea utilize a modified route for coenzyme A biosynthesis. PoK and PPS replace PS and PanK (used by bacteria and eukaryotes) in the conversion of pantoate to 4'-phosphopantothenate. See PubMed=31337720. |