HAMAP annotation rule MF_00156
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Accession MF_00156
Dates 1-JUN-2001 (Created)
9-NOV-2012 (Last updated, Version 27)
Name PanB
Scope Bacteria
Archaea
Triggered by



Identifier
PANB
Protein name
RecName: Full=3-methyl-2-oxobutanoate hydroxymethyltransferase;
EC=2.1.2.11;
AltName: Full=Ketopantoate hydroxymethyltransferase;
Short=KPHMT;
Gene name
panB


FUNCTION Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate (By similarity).
CATALYTIC ACTIVITY 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H(2)O = tetrahydrofolate + 2-dehydropantoate.
COFACTOR Binds 1 magnesium ion per subunit (By similarity).
PATHWAY Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate; step 1/2.
SUBUNIT Homodecamer; pentamer of dimers (By similarity).
SUBCELLULAR LOCATION Cytoplasm (Potential).
SIMILARITY Belongs to the PanB family.


Cytoplasm
Magnesium
Metal-binding
Methyltransferase
Pantothenate biosynthesis
Transferase


GO:0003864; Molecular function: 3-methyl-2-oxobutanoate hydroxymethyltransferase activity.
GO:0015940; Biological process: pantothenate biosynthetic process.
GO:0005737; Cellular component: cytoplasm.


Pfam PF02548; Pantoate_transf; 1;
PRINTS PR01415; ANKYRIN; 1;
TIGRFAMs TIGR00222; PanB; 1;


From: PANB_ECOLI (P31057)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     45     46       Alpha-ketoisovalerate binding (By similarity)        
ACT_SITE     181     181       Proton acceptor (By similarity)     E  
METAL     45     45       Magnesium (By similarity)     D  
METAL     84     84       Magnesium (By similarity)     D  
METAL     114     114       Magnesium (By similarity)     E  
BINDING     84     84       Alpha-ketoisovalerate (By similarity)     D  
BINDING     112     112       Alpha-ketoisovalerate (By similarity)     K  


Size range: 257-326 amino acids
Related Rules: None
Templates: P0A5Q8 (PANB_MYCTU); P31057 (PANB_ECOLI); Q9X712 (PANB_CORGL); Q9JZW6 (PANB_NEIMB): [Recover all]
Fusion: None


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