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HAMAP rule MF_00159
General rule information
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| Accession | MF_00159 |
| Dates | 18-JAN-2002 (Created)
28-JUN-2024 (Last updated, Version 40) |
| Name | IspG |
| Scope(s) |
Bacteria |
| Template(s) | P62620 (ISPG_ECOLI); Q84GJ3 (ISPG_THETH); O67496 (ISPG_AQUAE); Q72H18 (ISPG_THET2); [ Recover all ] |
| Triggered by |
HAMAP; MF_00159 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | ISPG |
| case <OC:Cyanobacteriota> | |
| Protein name | RecName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin); EC=1.17.7.1; AltName: Full=1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase; |
| else | |
| Protein name | RecName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin); EC=1.17.7.3; AltName: Full=1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase; |
| end case | |
| Gene name | Name=ispG; |
Comments
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| FUNCTION | Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME- 2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. |
| case <OC:Cyanobacteriota> or <OG:Chloroplast> | |
| CATALYTIC ACTIVITY | Reaction=(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:26119, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:58483, ChEBI:CHEBI:128753; EC=1.17.7.1; |
| else | |
| CATALYTIC ACTIVITY | Reaction=(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin]; Xref=Rhea:RHEA:43604, Rhea:RHEA- COMP:10622, Rhea:RHEA-COMP:10623, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:58483, ChEBI:CHEBI:128753; EC=1.17.7.3; |
| end case | |
| case <FTGroup:1> | |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster.; |
| end case | |
| PATHWAY | Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 5/6. |
| SIMILARITY | Belongs to the IspG family. |
Keywords
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| case <FTGroup:1> | |
| 4Fe-4S | |
| Metal-binding | |
| Iron | |
| Iron-sulfur | |
| end case | |
| Isoprene biosynthesis | |
| Oxidoreductase | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding | |
| end case | |
| GO:0046429; Molecular function:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity (ferredoxin) | |
| GO:0019288; Biological process:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway | |
Cross-references
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Features
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| From: ISPG_AQUAE (O67496) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 265 | 265 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 268 | 268 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 300 | 300 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 307 | 307 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
E | 1 | |||||||
Additional information
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| Size range | 344-663 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Insertion in BACFN, BACFR, BACTN, CHLAB, CHLCV, CHLMU, CHLPN, CHLTE, CHLTR, LEPIC, LEPIN, PARUW and PORGI. Possible wrong start in CHLTE, DESVH and RHOPA. |