HAMAP

Accession	MF_00159
Dates	18-JAN-2002 (Created) 18-MAY-2011 (Last updated, Version 28)

Data class

Protein

Predictors

HAMAP; MF_00159; [distribution of match scores in UniProtKB];[seed alignment for MF_00159]

Names

IspG

Identifier

ISPG

Protein name

RecName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase; EC=1.17.7.1; AltName: Full=1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase;

Gene name

ispG

FUNCTION: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (By similarity).

CATALYTIC ACTIVITY: (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H(2)O + 2 oxidized ferredoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin.

COFACTOR: Binds 1 4Fe-4S cluster (By similarity).

PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6.

SIMILARITY: Belongs to the IspG family.

Pfam	PF04551; GcpE; 1;
TIGRFAMs	TIGR00612; IspG_gcpE; 1;

4Fe-4S, Metal-binding, Iron, Iron-sulfur, Isoprene biosynthesis, Oxidoreductase.

GO:0046429; Molecular function: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity.
GO:0016114; Biological process: terpenoid biosynthetic process.

Size range:	344-663 amino acids
Related UniRules:	None
Template:	P62620 (ISPG_ECOLI); Q84GJ3 (ISPG_THETH): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in STRAW, STRCO
Plasmid encoded:	None
Comments:	Insertion in BACFN, BACFR, BACTN, CHLAB, CHLCV, CHLMU, CHLPN, CHLTE, CHLTR, LEPIC, LEPIN, PARUW and PORGI. Possible wrong start in CHLTE, DESVH and RHOPA.