HAMAP rule MF_00164
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00164 |
| Accession | MF_00164 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 42) |
| Name | GlmS |
| Scope(s) |
Bacteria Archaea Plastid |
| Template(s) | P17169 (GLMS_ECOLI); Q56213 (GLMS_THET8); Q6LWM9 (GLMS_METMP); [ Recover all ] |
| Triggered by |
HAMAP; MF_00164 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | GLMS |
| Protein name | RecName: Full=Glutamine--fructose-6-phosphate aminotransferase [isomerizing]; EC=2.6.1.16; AltName: Full=D-fructose-6-phosphate amidotransferase; AltName: Full=GFAT; AltName: Full=Glucosamine-6-phosphate synthase; AltName: Full=Hexosephosphate aminotransferase; AltName: Full=L-glutamine--D-fructose-6-phosphate amidotransferase; |
| Gene name | Name=glmS; |
Comments
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| FUNCTION | Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source. |
| CATALYTIC ACTIVITY | Reaction=D-fructose 6-phosphate + L-glutamine = D-glucosamine 6- phosphate + L-glutamate; Xref=Rhea:RHEA:13237, ChEBI:CHEBI:29985, ChEBI:CHEBI:58359, ChEBI:CHEBI:58725, ChEBI:CHEBI:61527; EC=2.6.1.16; |
| SUBUNIT | Homodimer. |
| case <OG:Chloroplast> | |
| SUBCELLULAR LOCATION | Plastid, chloroplast. |
| end case | |
| case not <OG:Chloroplast> | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| end case | |
Keywords
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| case not <OG:Chloroplast> | |
| Cytoplasm | |
| end case | |
| Transferase | |
| Aminotransferase | |
Gene Ontology
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| GO:0004360; Molecular function:glutamine-fructose-6-phosphate transaminase (isomerizing) activity | |
| GO:0005975; Biological process:carbohydrate metabolic process | |
| case <OG:Chloroplast> | |
| GO:0009507; Cellular component:chloroplast | |
| end case | |
| case not <OG:Chloroplast> | |
| GO:0005737; Cellular component:cytoplasm | |
| end case | |
Cross-references
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| Pfam | PF00310; GATase_2; 1; |
| Pfam | PF01380; SIS; 2; |
| NCBIfam | TIGR01135; GlmS; 1; |
| PROSITE | PS51278; GATASE_TYPE_2; 1; |
| PROSITE | PS51464; SIS; 1-2; |
Features
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| From: GLMS_ECOLI (P17169) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| INIT_MET | 1 | 1 | /note="Removed" | M | ||||||||
| ACT_SITE | 2 | 2 | /note="Nucleophile; for GATase activity" | C | ||||||||
| ACT_SITE | 604 | 604 | /note="For Fru-6P isomerization activity" | K | ||||||||
Additional information
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| Size range | 589-635 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Nodulating bacteria seem to have two copies: a glmS and a nodM. There does not seem to be any mean of distinguishing the two forms. Intein deleted in METJA (not shown in alignment). |