HAMAP rule MF_00171
General rule information
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| Accession | MF_00171 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 33) |
| Name | TruA |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P07649 (TRUA_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00171 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TRUA |
| Protein name | RecName: Full=tRNA pseudouridine synthase A; EC=5.4.99.12; AltName: Full=tRNA pseudouridine(38-40) synthase; AltName: Full=tRNA pseudouridylate synthase I; AltName: Full=tRNA-uridine isomerase I; |
| Gene name | Name=truA; |
Comments
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| FUNCTION | Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. |
| CATALYTIC ACTIVITY | Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA; Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12; |
| case <OC:Bacteria> | |
| SUBUNIT | Homodimer. |
| end case | |
| SIMILARITY | Belongs to the tRNA pseudouridine synthase TruA family. |
Keywords
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Gene Ontology
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| GO:0009982; Molecular function:pseudouridine synthase activity |
| GO:0031119; Biological process:tRNA pseudouridine synthesis |
Cross-references
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| Pfam | PF01416; PseudoU_synth_1; 1; |
| NCBIfam | TIGR00071; HisT_truA; 1; |
| PIRSF | PIRSF001430; tRNA_psdUrid_synth; 1; |
Features
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| From: TRUA_ECOLI (P07649) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 107 | 111 | /note="RNA binding" | F-H-A-R-F | 1 | |||||||
| REGION | 168 | 172 | /note="Interaction with tRNA" | Q-C-Q-S-R | 1 | |||||||
| ACT_SITE | 60 | 60 | /note="Nucleophile" | D | ||||||||
| BINDING | 118 | 118 | /ligand="substrate" | Y | ||||||||
| SITE | 58 | 58 | /note="Interaction with tRNA. Important for base-flipping" | R | 1 | |||||||
| SITE | 78 | 78 | /note="Interaction with tRNA" | R | 1 | |||||||
| SITE | 110 | 110 | /note="Interaction with tRNA" | R | 1 | |||||||
| SITE | 126 | 126 | /note="Interaction with tRNA" | R | 1 | |||||||
| SITE | 139 | 139 | /note="Interaction with tRNA" | F | 1 | |||||||
Additional information
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| Size range | 240-304 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Possible wrong start in MYCLE, MYCTU and MYCPA. Divergent LEPIN and NANEQ; not shown in alignment and not used in size range. |