HAMAP

RecName:	Full=tRNA pseudouridine synthase A; EC=5.4.99.12;
AltName:	Full=tRNA pseudouridine(38-40) synthase;
AltName:	Full=tRNA pseudouridylate synthase I;
AltName:	Full=tRNA-uridine isomerase I;

Gene name

truA

FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs (By similarity).

CATALYTIC ACTIVITY: tRNA uridine(38-40) = tRNA pseudouridine(38-40).

case <OC:Bacteria>

SUBUNIT: Homodimer (By similarity).

end case

SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.

Isomerase, tRNA processing.

GO:0009982; Molecular function: pseudouridine synthase activity.
GO:0031119; Biological process: tRNA pseudouridine synthesis.

`From: TRUA_ECOLI (P07649)`
`Key`	`From`	`To`	`Description`	`Condition`	`FTGroup`
`REGION (Optional)`	`107`	`111`	`RNA binding (By similarity)`	`F-H-A-R-F`	`1`
`REGION (Optional)`	`168`	`172`	`Interaction with tRNA (By similarity)`	`Q-C-Q-S-R`	`1`

`ACT_SITE`	`60`	`60`	`Nucleophile (By similarity)`	`D`
`BINDING`	`118`	`118`	`Substrate (By similarity)`	`Y`

`SITE (Optional)`	`58`	`58`	`Interaction with tRNA. Important for base-flipping (By similarity)`	`R`	`1`
`SITE (Optional)`	`78`	`78`	`Interaction with tRNA (By similarity)`	`R`	`1`
`SITE (Optional)`	`110`	`110`	`Interaction with tRNA (By similarity)`	`R`	`1`
`SITE (Optional)`	`126`	`126`	`Interaction with tRNA (By similarity)`	`R`	`1`
`SITE (Optional)`	`139`	`139`	`Interaction with tRNA (By similarity)`	`F`	`1`

Size range:	240-304 amino acids
Related UniRules:	None
Template:	P07649 (TRUA_ECOLI)
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	in BACAN, BACC1, BACCR, BACCZ, BACHK, CLOPE, CLOTE, DESPS, PARUW
Plasmid encoded:	None
Comments:	Possible wrong start in MYCLE, MYCTU and MYCPA. Divergent LEPIN and NANEQ; not shown in alignment and not used in size range.

UniProtKB rule member sequences

UniProtKB sets

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