HAMAP rule MF_00172
General rule information
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| Accession | MF_00172 |
| Dates | 1-JUN-2001 (Created)
28-NOV-2023 (Last updated, Version 35) |
| Name | Meth_synth |
| Scope(s) |
Bacteria |
| Template(s) | P25665 (METE_ECOLI); Q9X112 (METE_THEMA); Q8CWX6 (METE_STRMU); [ Recover all ] |
| Triggered by |
HAMAP; MF_00172 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | METE |
| Protein name | RecName: Full=5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; EC=2.1.1.14; AltName: Full=Cobalamin-independent methionine synthase; AltName: Full=Methionine synthase, vitamin-B12 independent isozyme; |
| Gene name | Name=metE; |
Comments
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| FUNCTION | Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation. |
| CATALYTIC ACTIVITY | Reaction=5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L- methionine + tetrahydropteroyltri-L-glutamate; Xref=Rhea:RHEA:21196, ChEBI:CHEBI:57844, ChEBI:CHEBI:58140, ChEBI:CHEBI:58199, ChEBI:CHEBI:58207; EC=2.1.1.14; |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| PATHWAY | Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. |
| SIMILARITY | Belongs to the vitamin-B12 independent methionine synthase family. |
Keywords
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| Amino-acid biosynthesis |
| Metal-binding |
| Methionine biosynthesis |
| Methyltransferase |
| Repeat |
| Transferase |
| Zinc |
Gene Ontology
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| GO:0003871; Molecular function:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity |
| GO:0009086; Biological process:methionine biosynthetic process |
Cross-references
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| Pfam | PF08267; Meth_synt_1; 1; |
| Pfam | PF01717; Meth_synt_2; 1; |
| PIRSF | PIRSF000382; MeTrfase_B12_ind; 1; |
| NCBIfam | TIGR01371; Met_syn_B12ind; 1; |
Features
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| From: METE_THEMA (Q9X112) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 15 | 18 | /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207" |
R-E-x-K | ||||||||
| BINDING | 409 | 411 | /ligand="L-homocysteine" /ligand_id="ChEBI:CHEBI:58199" |
I-G-S | ||||||||
| BINDING | 409 | 411 | /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844" |
I-G-S | ||||||||
| BINDING | 493 | 494 | /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207" |
R-C | ||||||||
| ACT_SITE | 672 | 672 | /note="Proton donor" | H | ||||||||
| BINDING | 618 | 618 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic" |
H | ||||||||
| BINDING | 620 | 620 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic" |
C | ||||||||
| BINDING | 642 | 642 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic" |
E | ||||||||
| BINDING | 704 | 704 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic" |
C | ||||||||
| BINDING | 104 | 104 | /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207" |
[KR] | ||||||||
| BINDING | 462 | 462 | /ligand="L-homocysteine" /ligand_id="ChEBI:CHEBI:58199" |
E | ||||||||
| BINDING | 462 | 462 | /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844" |
E | ||||||||
| BINDING | 539 | 539 | /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207" |
W | ||||||||
| BINDING | 577 | 577 | /ligand="L-homocysteine" /ligand_id="ChEBI:CHEBI:58199" |
D | ||||||||
| BINDING | 577 | 577 | /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844" |
D | ||||||||
| BINDING | 583 | 583 | /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207" |
E | ||||||||
Additional information
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| Size range | 734-792 amino acids |
| Related rules |
MF_00288 |
| Fusion | Nter: None Cter: None |