HAMAP rule MF_00176
General rule information
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Accession | MF_00176 |
Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 37) |
Name | Ser_tRNA_synth_type1 |
Scope(s) |
Bacteria Archaea |
Template(s) | P0A8L1 (SYS_ECOLI); P34945 (SYS_THET2); Q46F20 (SYS_METBF); P95689 (SYS_STAA8); P54221 (SYS_HALMA); [ Recover all ] |
Triggered by |
HAMAP; MF_00176 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | SYS |
Protein name | RecName: Full=Serine--tRNA ligase; EC=6.1.1.11; AltName: Full=Seryl-tRNA synthetase; Short=SerRS; AltName: Full=Seryl-tRNA(Ser/Sec) synthetase; |
Gene name | Name=serS; |
Comments
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FUNCTION | Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). |
CATALYTIC ACTIVITY | Reaction=ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L- seryl-tRNA(Ser); Xref=Rhea:RHEA:12292, Rhea:RHEA-COMP:9669, Rhea:RHEA-COMP:9703, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:33384, ChEBI:CHEBI:78442, ChEBI:CHEBI:78533, ChEBI:CHEBI:456215; EC=6.1.1.11; |
CATALYTIC ACTIVITY | Reaction=ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L- seryl-tRNA(Sec); Xref=Rhea:RHEA:42580, Rhea:RHEA-COMP:9742, Rhea:RHEA-COMP:10128, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:33384, ChEBI:CHEBI:78442, ChEBI:CHEBI:78533, ChEBI:CHEBI:456215; EC=6.1.1.11; |
PATHWAY | Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. |
SUBUNIT | Homodimer. The tRNA molecule binds across the dimer. |
SUBCELLULAR LOCATION | Cytoplasm. |
DOMAIN | Consists of two distinct domains, a catalytic core and a N- terminal extension that is involved in tRNA binding. |
SIMILARITY | Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily. |
Keywords
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Gene Ontology
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GO:0004828; Molecular function:serine-tRNA ligase activity |
GO:0005524; Molecular function:ATP binding |
GO:0016260; Biological process:selenocysteine biosynthetic process |
GO:0006434; Biological process:seryl-tRNA aminoacylation |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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PROSITE | PS50862; AA_TRNA_LIGASE_II; 1; |
Pfam | PF02403; Seryl_tRNA_N; 1; |
Pfam | PF00587; tRNA-synt_2b; 1; |
PRINTS | PR00981; TRNASYNTHSER; 1; |
NCBIfam | TIGR00414; SerS; 1; |
Features
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From: SYS_THET2 (P34945) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 256 | 258 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R-x-E | ||||||||
BINDING | 345 | 348 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
E-x-x-S | ||||||||
BINDING | 225 | 227 | /ligand="L-serine" /ligand_id="ChEBI:CHEBI:33384" |
T-x-E | ||||||||
BINDING | 272 | 272 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
V | ||||||||
BINDING | 279 | 279 | /ligand="L-serine" /ligand_id="ChEBI:CHEBI:33384" |
E | ||||||||
BINDING | 380 | 380 | /ligand="L-serine" /ligand_id="ChEBI:CHEBI:33384" |
[TS] |
Additional information
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Size range | 411-482 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |