Annotation rule MF_00176
Send feedback

General rule information [?]

Accession MF_00176
Dates 1-JUN-2001 (Created)
21-NOV-2011 (Last updated, Version 30)
Name Ser_tRNA_synth_type1
Scope Bacteria
Archaea
Templates P0A8L1 (SYS_ECOLI); P34945 (SYS_THET2); Q46F20 (SYS_METBF); P95689 (SYS_STAA8); P54221 (SYS_HALMA): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYS
Protein name
RecName: Full=Serine--tRNA ligase;
EC=6.1.1.11;
AltName: Full=Seryl-tRNA synthetase;
Short=SerRS;
AltName: Full=Seryl-tRNA(Ser/Sec) synthetase;
Gene name
serS

Comments [?]

Function Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (By similarity).
Catalytic activity ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).
Pathway Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec); step 1/1.
Subunit Homodimer. The tRNA molecule binds across the dimer (By similarity).
Subcellular location Cytoplasm (By similarity).
Domain Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding (By similarity).
Similarity Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Keywords [?]


Gene Ontology [?]

GO:0004828; Molecular function: serine-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0016260; Biological process: selenocysteine biosynthetic process.
GO:0006434; Biological process: seryl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS50862; AA_TRNA_LIGASE_II; 1;
Pfam PF02403; Seryl_tRNA_N; 1;
PF00587; tRNA-synt_2b; 1;
PRINTS PR00981; TRNASYNTHSER; 1;
TIGRFAMs TIGR00414; SerS; 1;

Features [?]

From: SYS_THET2 (P34945)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     256     258       ATP (By similarity)     R-x-E  
NP_BIND     345     348       ATP (By similarity)     E-x-x-S  
REGION     225     227       Serine binding (By similarity)     T-x-E  
BINDING (Optional)     272     272       ATP; via amide nitrogen and carbonyl oxygen (By similarity)     V  
BINDING     279     279       Serine (By similarity)     E  
BINDING     380     380       Serine (By similarity)     [TS]  

Additional information [?]

Size range 411-482 amino acids
Related rules None
Fusion None
Comments  There are two distinct types of seryl-tRNA synthetase, as differentiated by primary sequence analysis, three-dimensional structure and substrate recognition mechanism: type 1 (MF_00176) is found in the majority of organisms (prokaryotes, eukaryotes and archaea) whereas type 2 (MF_01278) is confined to some methanogenic archaea. METBF (Methanosarcina barkeri) possesses two seryl-tRNA synthetases, one of each type.