HAMAP rule MF_00180
General rule information
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| Accession | MF_00180 |
| Dates | 1-JUN-2001 (Created)
28-NOV-2023 (Last updated, Version 28) |
| Name | RibB |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0A7J0 (RIBB_ECOLI); Q60364 (RIBB_METJA); [ Recover all ] |
| Triggered by |
HAMAP; MF_00180 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RIBB |
| Protein name | RecName: Full=3,4-dihydroxy-2-butanone 4-phosphate synthase; Short=DHBP synthase; EC=4.1.99.12; |
| Gene name | Name=ribB; |
Comments
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| FUNCTION | Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. |
| CATALYTIC ACTIVITY | Reaction=D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+); Xref=Rhea:RHEA:18457, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:58121, ChEBI:CHEBI:58830; EC=4.1.99.12; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 2 divalent metal cations per subunit. Magnesium or manganese.; |
| PATHWAY | Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3- oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the DHBP synthase family. |
Keywords
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Gene Ontology
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| GO:0000287; Molecular function:magnesium ion binding |
| GO:0008686; Molecular function:3,4-dihydroxy-2-butanone-4-phosphate synthase activity |
| GO:0030145; Molecular function:manganese ion binding |
| GO:0009231; Biological process:riboflavin biosynthetic process |
Cross-references
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Features
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| From: RIBB_METJA (Q60364) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 25 | 26 | /ligand="D-ribulose 5-phosphate" /ligand_id="ChEBI:CHEBI:58121" |
R-E | ||||||||
| BINDING | 161 | 165 | /ligand="D-ribulose 5-phosphate" /ligand_id="ChEBI:CHEBI:58121" |
R-x-x-[HQ]-T | ||||||||
| BINDING | 26 | 26 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
E | ||||||||
| BINDING | 26 | 26 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
E | ||||||||
| BINDING | 164 | 164 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
H | ||||||||
| BINDING | 30 | 30 | /ligand="D-ribulose 5-phosphate" /ligand_id="ChEBI:CHEBI:58121" |
D | ||||||||
| SITE | 147 | 147 | /note="Essential for catalytic activity" | H | ||||||||
| SITE | 185 | 185 | /note="Essential for catalytic activity" | E | ||||||||
Additional information
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| Size range | 200-247 amino acids |
| Related rules |
MF_01283 |
| Fusion | Nter: None Cter: <ribA-like> |
| Comments | RibA and RibB are fused in some organisms (see MF_01283 for bifunctional RibBA). Some ribB are followed by a ribA-like domain, which does not seem to code for GTP cyclohydrolase II activity because it lacks all the residues important for activity. |