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HAMAP rule MF_00181

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General rule information [?]

Accession MF_00181
Dates 1-JUN-2001 (Created)
17-JAN-2023 (Last updated, Version 37)
Name Cytosol_peptidase_M17
Scope(s) Bacteria
Archaea
Template(s) P68767 (AMPA_ECOLI); P27888 (AMPA_RICPR); [ Recover all ]
Triggered by HAMAP; MF_00181 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier AMPA
Protein name RecName: Full=Probable cytosol aminopeptidase;
                 EC=3.4.11.1;
AltName: Full=Leucine aminopeptidase;
                 Short=LAP;
                 EC=3.4.11.10;
AltName: Full=Leucyl aminopeptidase;
Gene name Name=pepA;

Comments [?]

FUNCTIONPresumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N- terminal amino acids from various peptides.
CATALYTIC ACTIVITY Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1;
CATALYTIC ACTIVITY Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10;
COFACTOR Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 2 manganese ions per subunit.;
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the peptidase M17 family.

Keywords [?]


Gene Ontology [?]

GO:0004177; Molecular function:aminopeptidase activity
GO:0008235; Molecular function:metalloexopeptidase activity
GO:0030145; Molecular function:manganese ion binding
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00883; Peptidase_M17; 1;
PRINTS PR00481; LAMNOPPTDASE; 1;
PROSITE PS00631; CYTOSOL_AP; 1;

Features [?]

From: AMPA_ECOLI (P68767)
Key From To Description Tag Condition FTGroup
ACT_SITE 282 282 K
ACT_SITE 356 356 R
BINDING 270 270 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="2"
K
BINDING 275 275 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="1"
D
BINDING 275 275 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="2"
D
BINDING 293 293 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="2"
D
BINDING 352 352 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="1"
D
BINDING 354 354 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="1"
E
BINDING 354 354 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="2"
E

Additional information [?]

Size range 445-524 amino acids
Related rules MF_00504
Fusion Nter: None Cter: None
Comments Possible wrong C-terminal sequence in METSV



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