HAMAP rule MF_00181
General rule information
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| Accession | MF_00181 |
| Dates | 1-JUN-2001 (Created)
17-JAN-2023 (Last updated, Version 37) |
| Name | Cytosol_peptidase_M17 |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P68767 (AMPA_ECOLI); P27888 (AMPA_RICPR); [ Recover all ] |
| Triggered by |
HAMAP; MF_00181 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | AMPA |
| Protein name | RecName: Full=Probable cytosol aminopeptidase; EC=3.4.11.1; AltName: Full=Leucine aminopeptidase; Short=LAP; EC=3.4.11.10; AltName: Full=Leucyl aminopeptidase; |
| Gene name | Name=pepA; |
Comments
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| FUNCTION | Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N- terminal amino acids from various peptides. |
| CATALYTIC ACTIVITY | Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; |
| CATALYTIC ACTIVITY | Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; |
| COFACTOR | Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 2 manganese ions per subunit.; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the peptidase M17 family. |
Keywords
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Gene Ontology
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| GO:0004177; Molecular function:aminopeptidase activity |
| GO:0008235; Molecular function:metalloexopeptidase activity |
| GO:0030145; Molecular function:manganese ion binding |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: AMPA_ECOLI (P68767) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 282 | 282 | K | |||||||||
| ACT_SITE | 356 | 356 | R | |||||||||
| BINDING | 270 | 270 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2" |
K | ||||||||
| BINDING | 275 | 275 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1" |
D | ||||||||
| BINDING | 275 | 275 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2" |
D | ||||||||
| BINDING | 293 | 293 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2" |
D | ||||||||
| BINDING | 352 | 352 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1" |
D | ||||||||
| BINDING | 354 | 354 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1" |
E | ||||||||
| BINDING | 354 | 354 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2" |
E | ||||||||
Additional information
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| Size range | 445-524 amino acids |
| Related rules |
MF_00504 |
| Fusion | Nter: None Cter: None |
| Comments | Possible wrong C-terminal sequence in METSV |