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HAMAP rule MF_00191

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General rule information [?]

Accession MF_00191
Dates 1-JUN-2001 (Created)
28-JUN-2024 (Last updated, Version 50)
Name IspH
Scope(s) Bacteria
Template(s) P62623 (ISPH_ECOLI); O67625 (ISPH_AQUAE); Q55643 (ISPH_SYNY3); [ Recover all ]
Triggered by HAMAP; MF_00191 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ISPH
Protein name RecName: Full=4-hydroxy-3-methylbut-2-enyl diphosphate reductase;
                 Short=HMBPP reductase;
                 EC=1.17.7.4;
Gene name Name=ispH;

Comments [?]

FUNCTIONCatalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.
CATALYTIC ACTIVITY Reaction=H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]- [ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:24488, Rhea:RHEA- COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:128753, ChEBI:CHEBI:128769; EC=1.17.7.4;
CATALYTIC ACTIVITY Reaction=dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]- [ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:24825, Rhea:RHEA- COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57623, ChEBI:CHEBI:128753; EC=1.17.7.4;
case <FTGroup:1>
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per subunit.;
end case
PATHWAYIsoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3- methylbutenyl diphosphate: step 1/1.
PATHWAYIsoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 6/6.
case <OC:Enterobacterales>
SUBUNITHomodimer.
end case
SIMILARITYBelongs to the IspH family.

Keywords [?]

case <FTGroup:1>
4Fe-4S
Iron
Iron-sulfur
Metal-binding
end case
Isoprene biosynthesis
Oxidoreductase

Gene Ontology [?]

case <FTGroup:1>
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
end case
GO:0051745; Molecular function:4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
GO:0019288; Biological process:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
GO:0016114; Biological process:terpenoid biosynthetic process
GO:0050992; Biological process:dimethylallyl diphosphate biosynthetic process

Cross-references [?]

Pfam PF02401; LYTB; 1;
NCBIfam TIGR00216; IspH_lytB; 1;

Features [?]

From: ISPH_ECOLI (P62623)
Key From To Description Tag Condition FTGroup
ACT_SITE 126 126 /note="Proton donor" E
BINDING 12 12 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"		 C 1
BINDING 96 96 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"		 C 1
BINDING 197 197 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"		 C 1
BINDING 41 41 /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate"
/ligand_id="ChEBI:CHEBI:128753"		 H
BINDING 41 41 /ligand="dimethylallyl diphosphate"
/ligand_id="ChEBI:CHEBI:57623"		 H
BINDING 41 41 /ligand="isopentenyl diphosphate"
/ligand_id="ChEBI:CHEBI:128769"		 H
BINDING 74 74 /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate"
/ligand_id="ChEBI:CHEBI:128753"		 H
BINDING 74 74 /ligand="dimethylallyl diphosphate"
/ligand_id="ChEBI:CHEBI:57623"		 H
BINDING 74 74 /ligand="isopentenyl diphosphate"
/ligand_id="ChEBI:CHEBI:128769"		 H
BINDING 124 124 /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate"
/ligand_id="ChEBI:CHEBI:128753"		 H
BINDING 124 124 /ligand="dimethylallyl diphosphate"
/ligand_id="ChEBI:CHEBI:57623"		 H
BINDING 124 124 /ligand="isopentenyl diphosphate"
/ligand_id="ChEBI:CHEBI:128769"		 H
BINDING 167 167 /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate"
/ligand_id="ChEBI:CHEBI:128753"		 [TS]
BINDING 225 225 /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate"
/ligand_id="ChEBI:CHEBI:128753"		 S
BINDING 225 225 /ligand="dimethylallyl diphosphate"
/ligand_id="ChEBI:CHEBI:57623"		 S
BINDING 225 225 /ligand="isopentenyl diphosphate"
/ligand_id="ChEBI:CHEBI:128769"		 S
BINDING 226 226 /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate"
/ligand_id="ChEBI:CHEBI:128753"		 S
BINDING 226 226 /ligand="dimethylallyl diphosphate"
/ligand_id="ChEBI:CHEBI:57623"		 S
BINDING 226 226 /ligand="isopentenyl diphosphate"
/ligand_id="ChEBI:CHEBI:128769"		 S
BINDING 227 227 /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate"
/ligand_id="ChEBI:CHEBI:128753"		 N
BINDING 227 227 /ligand="dimethylallyl diphosphate"
/ligand_id="ChEBI:CHEBI:57623"		 N
BINDING 227 227 /ligand="isopentenyl diphosphate"
/ligand_id="ChEBI:CHEBI:128769"		 N
BINDING 269 269 /ligand="(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate"
/ligand_id="ChEBI:CHEBI:128753"		 S
BINDING 269 269 /ligand="dimethylallyl diphosphate"
/ligand_id="ChEBI:CHEBI:57623"		 S
BINDING 269 269 /ligand="isopentenyl diphosphate"
/ligand_id="ChEBI:CHEBI:128769"		 S

Additional information [?]

Size range 273-417 amino acids
Related rules None
Fusion Nter: None Cter: <S1>



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