HAMAP annotation rule MF_00191
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Accession MF_00191
Dates 1-JUN-2001 (Created)
24-APR-2012 (Last updated, Version 35)
Name IspH
Scope Bacteria
Triggered by



Identifier
ISPH
Protein name
RecName: Full=4-hydroxy-3-methylbut-2-enyl diphosphate reductase;
EC=1.17.1.2;
Gene name
ispH


FUNCTION Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) (By similarity).
CATALYTIC ACTIVITY Isopentenyl diphosphate + NAD(P)(+) + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H.
Dimethylallyl diphosphate + NAD(P)(+) + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H.
case <FTGroup:1>
COFACTOR Binds 1 3Fe-4S cluster per subunit (By similarity).
end case
PATHWAY Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate; step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate; step 6/6.
case <OC:Enterobacteriaceae>
SUBUNIT Homodimer (By similarity).
end case
SIMILARITY Belongs to the IspH family.


case <FTGroup:1>
3Fe-4S
Iron
Iron-sulfur
Metal-binding
end case
Isoprene biosynthesis
NADP
Oxidoreductase


case <FTGroup:1>
GO:0051538; Molecular function: 3 iron, 4 sulfur cluster binding.
end case
GO:0051745; Molecular function: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity.
GO:0019288; Biological process: isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway.
GO:0016114; Biological process: terpenoid biosynthetic process.


Pfam PF02401; LYTB; 1;
TIGRFAMs TIGR00216; IspH_lytB; 1;


From: ISPH_ECOLI (P62623)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     225     227       Substrate binding (By similarity)     S-x-N  
METAL     12     12       Iron-sulfur (3Fe-4S) (By similarity)     C   1
METAL     96     96       Iron-sulfur (3Fe-4S) (By similarity)     C   1
METAL     197     197       Iron-sulfur (3Fe-4S) (By similarity)     C   1
BINDING     41     41       Substrate (By similarity)     H  
BINDING     74     74       Substrate (By similarity)     [HF]  
BINDING     124     124       Substrate (By similarity)     H  
BINDING     167     167       Substrate (By similarity)     [TS]  
BINDING     269     269       Substrate (By similarity)     S  


Size range: 273-417 amino acids
Related Rules: None
Templates: P62623 (ISPH_ECOLI); O67625 (ISPH_AQUAE); Q55643 (ISPH_SYNY3): [Recover all]
Fusion: Nter: None; Cter: <S1>


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