HAMAP rule MF_00198
General rule information
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| Accession | MF_00198 |
| Dates | 1-JUN-2001 (Created)
2-SEP-2024 (Last updated, Version 48) |
| Name | Spermidine_synth |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P09158 (SPEE_ECOLI); P70998 (SPEE_BACSU); Q9WZC2 (SPEE_THEMA); Q8U4G1 (SPEE_PYRFU); O25503 (SPEE_HELPY); O57950 (SPEE_PYRHO); Q9UXE4 (SPEE_SACS2); Q5JFG9 (SPEE_THEKO); Q5SK28 (SPEE_THET8); [ Recover all ] |
| Triggered by |
HAMAP; MF_00198 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SPEE |
| Protein name | RecName: Full=Polyamine aminopropyltransferase; AltName: Full=Spermidine synthase; Short=SPDSY; Short=SPDS; EC=2.5.1.16; AltName: Full=Putrescine aminopropyltransferase; Short=PAPT; |
| Gene name | Name=speE; |
Comments
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| FUNCTION | Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine. |
| CATALYTIC ACTIVITY | Reaction=S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S- methyl-5'-thioadenosine + spermidine + H(+); Xref=Rhea:RHEA:12721, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:57443, ChEBI:CHEBI:57834, ChEBI:CHEBI:326268; EC=2.5.1.16; |
| PATHWAY | Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1. |
| case <Feature:Transmembrane>1> | |
| SUBCELLULAR LOCATION | Cell membrane; Multi-pass membrane protein. |
| else case <Feature:Transmembrane==1> | |
| SUBCELLULAR LOCATION | Cell membrane; Single-pass membrane protein. |
| else | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| end case | |
| SUBUNIT | Homodimer or homotetramer. |
| SIMILARITY | Belongs to the spermidine/spermine synthase family. |
| case not <FTTag:Act_site> | |
| CAUTION | Lacks the conserved Asp active site. |
| end case | |
Keywords
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| Polyamine biosynthesis | |
| Spermidine biosynthesis | |
| Transferase | |
| case <Feature:Transmembrane>1> | |
| Cell membrane | |
| Membrane | |
| Transmembrane | |
| Transmembrane helix | |
| else | |
| Cytoplasm | |
| end case | |
Gene Ontology
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| GO:0004766; Molecular function:spermidine synthase activity |
| GO:0008295; Biological process:spermidine biosynthetic process |
Cross-references
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| Pfam | PF01564; Spermine_synth; 1; |
| NCBIfam | TIGR00417; SpeE; 1; |
| PROSITE | PS01330; PABS_1; 1; |
| PROSITE | PS51006; PABS_2; 1; |
| General | Transmembrane; -; 7; |
Features
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| From: SPEE_PYRHO (O57950) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 144 | 145 | /ligand="S-methyl-5'-thioadenosine" /ligand_id="ChEBI:CHEBI:17509" |
[DNE]-[AGV] | ||||||||
| BINDING | 161 | 164 | /ligand="spermidine" /ligand_id="ChEBI:CHEBI:57834" |
D-[SC]-x-[DNE] | ||||||||
| ACT_SITE | 161 | 161 | /note="Proton acceptor" | Act_site | D | |||||||
| BINDING | 32 | 32 | /ligand="S-methyl-5'-thioadenosine" /ligand_id="ChEBI:CHEBI:17509" |
[QNR] | ||||||||
| BINDING | 63 | 63 | /ligand="spermidine" /ligand_id="ChEBI:CHEBI:57834" |
H | ||||||||
| BINDING | 87 | 87 | /ligand="spermidine" /ligand_id="ChEBI:CHEBI:57834" |
[DNE] | ||||||||
| BINDING | 107 | 107 | /ligand="S-methyl-5'-thioadenosine" /ligand_id="ChEBI:CHEBI:17509" |
[ED] | ||||||||
| BINDING | 168 | 168 | /ligand="S-methyl-5'-thioadenosine" /ligand_id="ChEBI:CHEBI:17509" |
P | ||||||||
Additional information
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| Size range | 261-574 amino acids |
| Related rules |
None |
| Fusion | Nter: <Unknown> Cter: <Unknown> |