HAMAP

Accession	MF_00200
Dates	1-JUN-2001 (Created) 19-OCT-2010 (Last updated, Version 20)

Data class

Protein

Predictors

HAMAP; MF_00200; [distribution of match scores in UniProtKB];[seed alignment for MF_00200]

Names

RTC

Identifier

RTCA

Protein name

RecName: Full=RNA 3'-terminal phosphate cyclase; Short=RNA cyclase; Short=RNA-3'-phosphate cyclase; EC=6.5.1.4;

Gene name

rtcA

FUNCTION: Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).

CATALYTIC ACTIVITY: ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.

SUBCELLULAR LOCATION: Cytoplasm (Potential).

SIMILARITY: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

PROSITE	PS01287; RTC; 1;
Pfam	PF01137; RTC; 1;
TIGRFAMs	TIGR03399; RNA_3prim_cycl; 1;

ATP-binding, Cytoplasm, Ligase, Nucleotide-binding.

GO:0003963; Molecular function: RNA-3'-phosphate cyclase activity.
GO:0005737; Cellular component: cytoplasm.

From: RTCA_ECOLI (P46849)
Key		From		To		Description		Condition		FTGroup
NP_BIND		283		287		ATP (By similarity)		[YHF]-x-x-D-[QH]
ACT_SITE		308		308		Tele-AMP-histidine intermediate (By similarity)		H
BINDING		103		103		ATP (By similarity)		[QD]

Size range:	328-361 amino acids
Related UniRules:	None
Template:	P46849 (RTCA_ECOLI)
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None