HAMAP rule MF_00200

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_00200
Accession	MF_00200
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 30)
Name	RTC
Scope(s)	Bacteria Archaea
Template(s)	P46849 (RTCA_ECOLI); [ Recover all ]
Triggered by	HAMAP; MF_00200 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	RTCA
Protein name	RecName: Full=RNA 3'-terminal phosphate cyclase; Short=RNA cyclase; Short=RNA-3'-phosphate cyclase; EC=6.5.1.4;
Gene name	Name=rtcA;

Comments [?]

FUNCTION	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
CATALYTIC ACTIVITY	Reaction=a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'- cyclophospho-ribonucleotide-RNA + AMP + diphosphate; Xref=Rhea:RHEA:23976, Rhea:RHEA-COMP:10463, Rhea:RHEA-COMP:10464, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:83062, ChEBI:CHEBI:83064, ChEBI:CHEBI:456215; EC=6.5.1.4;
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003963; Molecular function:RNA-3'-phosphate cyclase activity

GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Features [?]

From: RTCA_ECOLI (P46849)

Key

From

Description

Tag

Condition

FTGroup

BINDING

283

287

/ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"

[YHF]-x-x-D-[QH]

ACT_SITE

308

/note="Tele-AMP-histidine intermediate"

BINDING

103

/ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"

[QD]

Additional information [?]

Size range	319-400 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	Generally, RNA 3'-terminal phosphate cyclases are ATP dependent, but P.furiosus enzyme preferentially uses GTP rather than ATP (PubMed:22074260).