HAMAP

Accession	MF_00204
Dates	1-JUN-2001 (Created) 18-AUG-2011 (Last updated, Version 31)

Data class

Protein

Predictors

HAMAP; MF_00204; [distribution of match scores in UniProtKB];[seed alignment for MF_00204]

Names

UvrB

Identifier

UVRB

Protein name

RecName: Full=UvrABC system protein B; Short=Protein UvrB; AltName: Full=Excinuclease ABC subunit B;

Gene name

uvrB

FUNCTION: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage (By similarity).

SUBUNIT: Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

DOMAIN: The beta-hairpin motif is involved in DNA binding (By similarity).

SIMILARITY: Belongs to the UvrB family.

SIMILARITY: Contains 1 helicase ATP-binding domain.

SIMILARITY: Contains 1 helicase C-terminal domain.

SIMILARITY: Contains 1 UVR domain.

Pfam	PF00271; Helicase_C; 1; PF04851; ResIII; 1; PF02151; UVR; 1;
TIGRFAMs	TIGR00631; Uvrb; 1;
PROSITE	PS51192; HELICASE_ATP_BIND_1; 1; trigger=PRU00541; PS51194; HELICASE_CTER; 1; trigger=PRU00542; PS50151; UVR; 0-1; trigger=PRU00217;

Cytoplasm, DNA damage, SOS response, Excision nuclease, DNA repair, DNA excision, ATP-binding, Nucleotide-binding.

GO:0005524; Molecular function: ATP binding.
GO:0003677; Molecular function: DNA binding.
GO:0009381; Molecular function: excinuclease ABC activity.
GO:0006281; Biological process: DNA repair.
GO:0006289; Biological process: nucleotide-excision repair.
GO:0009432; Biological process: SOS response.
GO:0005737; Cellular component: cytoplasm.

From: UVRB_ECOLI (P0A8F8)
Key		From		To		Description		Condition		FTGroup
NP_BIND		39		46		ATP (Potential)		G-[IVAT]-T-G-[ST]-G-K-[ST]
MOTIF		92		115		Beta-hairpin

Size range:	645-709 amino acids
Related UniRules:	None
Template:	P56981 (UVRB_BACCA); P0A8F8 (UVRB_ECOLI); Q56243 (UVRB_THET8): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None