Annotation rule MF_00219
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General rule information [?]

Accession MF_00219
Dates 1-JUN-2001 (Created)
5-MAY-2017 (Last updated, Version 34)
Name PyrC_classII
Scope Bacteria
Templates P05020 (PYRC_ECOLI); A6T7D6 (PYRC_KLEP7); B1IV40 (PYRC_ECOLC); P06204 (PYRC_SALTY); Q8ZFU4 (PYRC_YERPE): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PYRC
Protein name
RecName: Full=Dihydroorotase;
Short=DHOase;
EC=3.5.2.3;
Gene name
pyrC

Comments [?]

Function Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.
Catalytic activity (S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor Zn(2+)
Note: Binds 2 Zn(2+) ions per subunit.
Pathway Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.
Subunit Homodimer.
Similarity Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.

Keywords [?]


Gene Ontology [?]

GO:0004151; Molecular function: dihydroorotase activity.
GO:0008270; Molecular function: zinc ion binding.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.

Cross-references [?]

Pfam PF01979; Amidohydro_1; 1;
PIRSF PIRSF001237; DHOdimr; 1;
TIGRFAMs TIGR00856; PyrC_dimer; 1;
PROSITE PS00482; DIHYDROOROTASE_1; 1;
PS00483; DIHYDROOROTASE_2; 1;

Features [?]

From: PYRC_ECOLI (P05020)
Key     From     To       Description   Tag   Condition   FTGroup
REGION (Optional)     19     21       Substrate binding     H-[LFV]-R  
ACT_SITE     251     251             D  
METAL     17     17       Zinc 1; via tele nitrogen     H  
METAL     19     19       Zinc 1; via tele nitrogen     H  
METAL     103     103       Zinc 1; via carbamate group     K  
METAL     103     103       Zinc 2; via carbamate group     K  
METAL     140     140       Zinc 2; via pros nitrogen     H  
METAL     178     178       Zinc 2; via tele nitrogen     H  
METAL     251     251       Zinc 1     D  
BINDING (Optional)     45     45       Substrate     N  
BINDING (Optional)     140     140       Substrate     H  
BINDING (Optional)     223     223       Substrate; via amide nitrogen and carbonyl oxygen     L  
BINDING (Optional)     255     255       Substrate     H  
BINDING (Optional)     267     267       Substrate; via carbonyl oxygen     A  
MOD_RES     103     103       N6-carboxylysine     K  

Additional information [?]

Size range 300-400 amino acids
Related rules MF_00220 (PYRC)
Fusion None
Comments Classification into subfamilies was done according to PubMed:24332717