HAMAP rule MF_00222
General rule information
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| Accession | MF_00222 |
| Dates | 1-JUN-2001 (Created)
12-MAR-2024 (Last updated, Version 42) |
| Name | Shikimate_DH_AroE |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P15770 (AROE_ECOLI); O67049 (AROE_AQUAE); P43876 (AROE_HAEIN); P56119 (AROE_HELPY); Q8Y9N5 (AROE_LISMO); Q5HNV1 (AROE_STAEQ); Q5SJF8 (AROE_THET8); Q9KVT3 (AROE_VIBCH); Q58484 (AROE_METJA); P44774 (SHDHL_HAEIN); Q9X5C9 (AROE_CORGL); [ Recover all ] |
| Triggered by |
HAMAP; MF_00222 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | AROE |
| Protein name | RecName: Full=Shikimate dehydrogenase (NADP(+)); Short=SDH; EC=1.1.1.25; |
| Gene name | Name=aroE; |
Comments
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| FUNCTION | Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA). |
| CATALYTIC ACTIVITY | Reaction=NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH; Xref=Rhea:RHEA:17737, ChEBI:CHEBI:15378, ChEBI:CHEBI:16630, ChEBI:CHEBI:36208, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.25; |
| PATHWAY | Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the shikimate dehydrogenase family. |
Keywords
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Gene Ontology
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| GO:0004764; Molecular function:shikimate 3-dehydrogenase (NADP+) activity |
| GO:0009423; Biological process:chorismate biosynthetic process |
Cross-references
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Features
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| From: AROE_AQUAE (O67049) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 130 | 134 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
G-[AGNSVY]-G-G-[AISV] | ||||||||
| BINDING | 153 | 158 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
N-R-T-x(2)-[KR] | ||||||||
| BINDING | 19 | 21 | /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208" |
[ST]-x-[ST] | ||||||||
| ACT_SITE | 70 | 70 | /note="Proton acceptor" | K | ||||||||
| BINDING | 82 | 82 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
[DE] | ||||||||
| BINDING | 66 | 66 | /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208" |
[TS] | ||||||||
| BINDING | 91 | 91 | /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208" |
N | ||||||||
| BINDING | 106 | 106 | /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208" |
D | ||||||||
| BINDING | 214 | 214 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
[IVMLCATFG] | ||||||||
| BINDING | 216 | 216 | /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208" |
Y | ||||||||
| BINDING | 235 | 235 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
G | ||||||||
| BINDING | 242 | 242 | /ligand="shikimate" /ligand_id="ChEBI:CHEBI:36208" |
Q | ||||||||
Additional information
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| Size range | 253-295 amino acids |
| Related rules |
MF_01578 MF_03143 |
| Fusion | Nter: MF_00214 (aroD) Cter: None |
| Comments | Aroe in CORGL and CORGB catalyzes the NAD(+)-dependent oxidation of both quinate and shikimate with a higher catalytic efficiency for quinate rather than shikimate, and seems to play a key role in the quinate-degradation pathway (PubMed=18566515). |