HAMAP

Accession	MF_00225
Dates	1-JUN-2001 (Created) 19-MAY-2011 (Last updated, Version 35)

Data class

Protein

Predictors

HAMAP; MF_00225; [distribution of match scores in UniProtKB];[seed alignment for MF_00225]

Names

DHO_dh_type2

Identifier

PYRD

Protein name

RecName: Full=Dihydroorotate dehydrogenase (quinone); EC=1.3.5.2; AltName: Full=DHOdehase; Short=DHOD; Short=DHODase; AltName: Full=Dihydroorotate oxidase;

Gene name

pyrD

FUNCTION: Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor (By similarity).

CATALYTIC ACTIVITY: (S)-dihydroorotate + a quinone = orotate + a quinol.

COFACTOR: Binds 1 FMN per subunit (By similarity).

PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.

SUBUNIT: Monomer (By similarity).

SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein (By similarity).

SIMILARITY: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Pfam	PF01180; DHO_dh; 1;
PIRSF	PIRSF000164; DHO_oxidase; 1;
TIGRFAMs	TIGR01036; PyrD_sub2; 1;
PROSITE	PS00911; DHODEHASE_1; 1; PS00912; DHODEHASE_2; 1;

Cell membrane, Flavoprotein, FMN, Membrane, Oxidoreductase, Pyrimidine biosynthesis.

GO:0004152; Molecular function: dihydroorotate dehydrogenase activity.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.
GO:0005886; Cellular component: plasma membrane.

From: PYRD_ECOLI (P0A7E1)
Key		From		To		Description		Condition		FTGroup
NP_BIND		62		66		FMN (By similarity)		[AP]-x-x-x-K
NP_BIND		318		319		FMN (By similarity)		[YF]-[ST]
REGION		111		115		Substrate binding (By similarity)		N-x-[MYLFN]-G-[FL]
REGION		246		247		Substrate binding (By similarity)		N-T
ACT_SITE		175		175		Nucleophile (By similarity)		[SC]
BINDING		66		66		Substrate (By similarity)		K
BINDING		86		86		FMN; via amide nitrogen (By similarity)		[TASG]
BINDING		139		139		FMN (By similarity)		[NS]
BINDING		172		172		FMN (By similarity)		N
BINDING		172		172		Substrate (By similarity)		N
BINDING		177		177		Substrate (By similarity)		N
BINDING		217		217		FMN (By similarity)		K
BINDING		245		245		FMN; via carbonyl oxygen (By similarity)		[TASGV]
BINDING		268		268		FMN; via amide nitrogen (By similarity)		G
BINDING		297		297		FMN; via amide nitrogen (By similarity)		G

Size range:	333-396 amino acids
Related UniRules:	MF_00224 (PYRD)
Template:	P0A7E1 (PYRD_ECOLI)
Scope:	Archaea; Halobacteriales Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	in CORST