HAMAP

Accession	MF_00228
Dates	1-JUN-2001 (Created) 19-MAY-2011 (Last updated, Version 34)

Data class

Protein

Predictors

HAMAP; MF_00228; [distribution of match scores in UniProtKB];[seed alignment for MF_00228]

Names

Thz_kinase

Identifier

THIM

Protein name

RecName: Full=Hydroxyethylthiazole kinase; EC=2.7.1.50; AltName: Full=4-methyl-5-beta-hydroxyethylthiazole kinase; Short=TH kinase; Short=Thz kinase;

Gene name

thiM

CATALYTIC ACTIVITY: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.

COFACTOR: Magnesium (By similarity).

PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1.

SIMILARITY: Belongs to the Thz kinase family.

Pfam	PF02110; HK; 1;
PRINTS	PR01099; HYETHTZKNASE; 1;
TIGRFAMs	TIGR00694; ThiM; 1;

Magnesium, Metal-binding, Thiamine biosynthesis, Transferase, Kinase, ATP-binding, Nucleotide-binding.

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0004417; Molecular function: hydroxyethylthiazole kinase activity.

From: THIM_BACSU (P39593)
Key		From		To		Description		Condition		FTGroup
BINDING		45		45		Substrate; via amide nitrogen (By similarity)		M
BINDING		121		121		ATP (By similarity)
BINDING		168		168		ATP (By similarity)		[ST]
BINDING		195		195		Substrate; via amide nitrogen (By similarity)		[GA]

Size range:	250-274 amino acids
Related UniRules:	None
Template:	P39593 (THIM_BACSU)
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: MF_00097 (thiE)
Duplicate:	in CLOB1, CLOB6, CLOBH, CLOBJ, CLOBK, CLOBL, CLOBM, LEUMM, METST, OCEIH, STRP2, STRP4, STRP7, STRPI, STRPJ, STRPN, STRPS, STRR6, STRZJ, STRZP, STRZT
Plasmid encoded:	in RHIE6, RHIEC, RHIL3, RHILW
Comments:	AGRT5 has internal deletion; not shown in alignment or used in size range. Fusion with thiE in SYMTH.