HAMAP

Accession	MF_00248
Dates	1-JUN-2001 (Created) 1-MAR-2011 (Last updated, Version 30)

Data class

Protein

Predictors

HAMAP; MF_00248; [distribution of match scores in UniProtKB];[seed alignment for MF_00248]

Names

HslV

Identifier

HSLV

Protein name

RecName: Full=ATP-dependent protease subunit HslV; EC=3.4.25.2; AltName: Full=Heat shock protein HslV;

Protein name

RecName: Full=ATP-dependent protease subunit HslV; EC=3.4.25.2; AltName: Full=Heat shock protein HslV;

Protein name

RecName: Full=ATP-dependent protease subunit HslV; EC=3.4.25.2;

Gene name

hslV

FUNCTION: Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery (By similarity).

CATALYTIC ACTIVITY: ATP-dependent cleavage of peptide bonds with broad specificity.

ENZYME REGULATION: Allosterically activated by HslU binding (By similarity).

SUBUNIT: A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

INDUCTION: By heat shock (By similarity).

SIMILARITY: Belongs to the peptidase T1B family. HslV subfamily.

Pfam	PF00227; Proteasome; 1;

Allosteric enzyme, Cytoplasm, Hydrolase, Protease.

Metal-binding, Sodium.

Stress response.

Threonine protease.

GO:0008233; Molecular function: peptidase activity.
GO:0030163; Biological process: protein catabolic process.
GO:0005737; Cellular component: cytoplasm.
GO:0009376; Cellular component: HslUV protease complex.

From: HSLV_ECOLI (P0A7B8)
Key		From		To		Description		Condition		FTGroup
ACT_SITE		2		2		By similarity		T
METAL (Optional)		157		157		Sodium; via carbonyl oxygen (By similarity)		[GAS]
METAL		160		160		Sodium; via carbonyl oxygen (By similarity)		[CD]
METAL		163		163		Sodium; via carbonyl oxygen (By similarity)		[TS]

Size range:	173-193 amino acids
Related UniRules:	None
Template:	P0A7B8 (HSLV_ECOLI); P43772 (HSLV_HAEIN); P39070 (CLPQ_BACSU); Q9WYZ1 (HSLV_THEMA): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	The ATPase subunit of the HslUV complex is described in MF_00249. The family member in B.subtilis (ClpQ) was shown to be a serine protease (EC=3.4.21.-) and not a threonine protease (EC=3.4.25.-). The N-terminus with the active site Ser is conserved only in some bacilli. The threonine (or serine) active site should be at the first position in the mature protein, so each protein should contain a FT INIT_MET or FT PROPEP, but this is not currently possible to make the rule manage this.