HAMAP

Accession	MF_00249
Dates	1-JUN-2001 (Created) 9-JUN-2011 (Last updated, Version 27)

Data class

Protein

Predictors

HAMAP; MF_00249; [distribution of match scores in UniProtKB];[seed alignment for MF_00249]

Names

HslU

Identifier

HSLU

Protein name

RecName: Full=ATP-dependent protease ATPase subunit HslU; AltName: Full=Heat shock protein HslU; AltName: Full=Unfoldase HslU;

Protein name

RecName: Full=ATP-dependent protease ATPase subunit HslU; AltName: Full=Unfoldase HslU;

Gene name

hslU

FUNCTION: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis (By similarity).

SUBUNIT: A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

INDUCTION: By heat shock (By similarity).

SIMILARITY: Belongs to the ClpX chaperone family. HslU subfamily.

Pfam	PF00004; AAA; 1; PF07724; AAA_2; 1; PF10431; ClpB_D2-small; 1;
TIGRFAMs	TIGR00390; HslU; 1;

Cytoplasm, ATP-binding, Chaperone.

Stress response.

Nucleotide-binding.

GO:0005524; Molecular function: ATP binding.
GO:0016887; Molecular function: ATPase activity.
GO:0043335; Biological process: protein unfolding.
GO:0005737; Cellular component: cytoplasm.
GO:0009376; Cellular component: HslUV protease complex.

From: HSLU_ECOLI (P0A6H5)
Key		From		To		Description		Condition		FTGroup
NP_BIND		60		65		ATP (By similarity)		G-[VIC]-G-K-T-E
BINDING		18		18		ATP; via amide nitrogen and carbonyl oxygen (By similarity)		[IV]
BINDING		256		256		ATP (By similarity)		D
BINDING		321		321		ATP (By similarity)		E
BINDING		393		393		ATP (By similarity)		R

Size range:	430-491 amino acids
Related UniRules:	MF_00175 (CLPX)
Template:	P0A6H5 (HSLU_ECOLI); P43773 (HSLU_HAEIN); P39778 (CLPY_BACSU); Q9WYZ2 (HSLU_THEMA): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	The protease subunit of the HslUV complex is described in MF_00248. Unlike in E.coli, H.influenzae and T.maritima, the family member in B.subtilis (ClpY) was shown to be part of a complex (ClpQY) with serine protease activity and not threonine protease activity.