Annotation rule MF_00277
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General rule information [?]

Accession MF_00277
Dates 1-JUN-2001 (Created)
10-OCT-2014 (Last updated, Version 25)
Name PII_uridylyl_transf
Scope Bacteria
Templates P27249 (GLND_ECOLI); Q8RQD1 (GLND_AZOBR); P36223 (GLND_AZOVI); P9WN29 (GLND_MYCTU); P41393 (GLND_KLEOX); Q9RAE4 (GLND_RHILV): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
GLND
Protein name
RecName: Full=Bifunctional uridylyltransferase/uridylyl-removing enzyme;
Short=UTase/UR;
AltName: Full=Bifunctional nitrogen sensor protein;
AltName: Full=Bifunctional [protein-PII] modification enzyme;
Includes:
RecName: Full=[Protein-PII] uridylyltransferase;
Short=PII uridylyltransferase;
Short=UTase;
EC=2.7.7.59;
Includes:
RecName: Full=[Protein-PII]-UMP uridylyl-removing enzyme;
Short=UR;
EC=3.1.4.-;
Gene name
glnD

Comments [?]

case defined <Property:NITROGEN_FIXATION> and <Property:NITROGEN_FIXATION>
Function Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism.
else case defined <Property:NITROGEN_FIXATION> and not <Property:NITROGEN_FIXATION>
Function Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.
else
Function Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen metabolism.
end case
Catalytic activity UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].
Uridylyl-[protein-PII] + H(2)O = UMP + [protein-PII].
Cofactor Mg(2+)
Enzyme regulation Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.
Domain Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.
Similarity Belongs to the GlnD family.

Keywords [?]

case <Property:NITROGEN_FIXATION>
end case

Gene Ontology [?]

GO:0008773; Molecular function: [protein-PII] uridylyltransferase activity.
GO:0008081; Molecular function: phosphoric diester hydrolase activity.
GO:0006808; Biological process: regulation of nitrogen utilization.
case <Property:NITROGEN_FIXATION>
GO:0009399; Biological process: nitrogen fixation.
end case

Cross-references [?]

PROSITE PS51671; ACT; 1; trigger=PRU01007;
Pfam PF01842; ACT; 1;
PF01966; HD; 1; trigger=Yes;
PF01909; NTP_transf_2; 1;
PIRSF PIRSF006288; PII_uridyltransf; 1;
TIGRFAMs TIGR01693; UTase_glnD; 1;

Features [?]

From: GLND_ECOLI (P27249)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     349       Uridylyltransferase        
REGION     350     708       Uridylyl-removing        

Additional information [?]

Size range 808-949 amino acids
Related rules None
Fusion None
Comments Divergent CORGL not shown in alignment and not used in size range