HAMAP

Accession	MF_00279
Dates	1-JUN-2001 (Created) 9-JUN-2011 (Last updated, Version 24)

Data class

Protein

Predictors

HAMAP; MF_00279; [distribution of match scores in UniProtKB];[seed alignment for MF_00279]

Names

PdxJ

Identifier

PDXJ

Protein name

RecName: Full=Pyridoxine 5'-phosphate synthase; Short=PNP synthase; EC=2.6.99.2;

Gene name

pdxJ

FUNCTION: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate (By similarity).

CATALYTIC ACTIVITY: 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H(2)O.

PATHWAY: Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 5/5.

SUBUNIT: Homooctamer; tetramer of dimers (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the PNP synthase family.

Pfam	PF03740; PdxJ; 1;
TIGRFAMs	TIGR00559; PdxJ; 1;

Cytoplasm, Pyridoxine biosynthesis, Transferase.

GO:0016769; Molecular function: transferase activity, transferring nitrogenous groups.
GO:0008615; Biological process: pyridoxine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

From: PDXJ_ECOLI (P0A794)
Key		From		To		Description		Condition		FTGroup
REGION (Optional)		11		12		1-deoxy-D-xylulose 5-phosphate binding (By similarity)		D-H
REGION		215		216		3-amino-2-oxopropyl phosphate binding (By similarity)		G-[HQFY]
ACT_SITE		45		45		Proton acceptor (By similarity)		H
ACT_SITE		72		72		Proton acceptor (By similarity)		E
ACT_SITE		193		193		Proton donor (By similarity)		H
BINDING		9		9		3-amino-2-oxopropyl phosphate (By similarity)		N
BINDING		20		20		3-amino-2-oxopropyl phosphate (By similarity)		R
BINDING		47		47		1-deoxy-D-xylulose 5-phosphate (By similarity)		R
BINDING		52		52		1-deoxy-D-xylulose 5-phosphate (By similarity)		H
BINDING		102		102		1-deoxy-D-xylulose 5-phosphate (By similarity)		T
BINDING (Optional)		194		194		3-amino-2-oxopropyl phosphate; via amide nitrogen (By similarity)		[GASD]
SITE		153		153		Transition state stabilizer (By similarity)		E

Size range:	232-267 amino acids
Related UniRules:	None
Template:	P0A794 (PDXJ_ECOLI)
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None