HAMAP

Accession	MF_00281
Dates	1-JUN-2001 (Created) 21-NOV-2011 (Last updated, Version 24)

Data class

Protein

Predictors

HAMAP; MF_00281; [distribution of match scores in UniProtKB];[seed alignment for MF_00281]

Names

Phe_tRNA_synth_alpha1

Identifier

SYFA

Protein name

RecName: Full=Phenylalanine--tRNA ligase alpha chain; EC=6.1.1.20; AltName: Full=Phenylalanyl-tRNA synthetase alpha chain; Short=PheRS;

Gene name

pheS

CATALYTIC ACTIVITY: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

COFACTOR: Binds 2 magnesium ions per tetramer (By similarity).

SUBUNIT: Tetramer of two alpha and two beta chains (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Pfam	PF01409; tRNA-synt_2d; 1;
TIGRFAMs	TIGR00468; PheS; 1;
PROSITE	PS50862; AA_TRNA_LIGASE_II; 1;

Cytoplasm, Aminoacyl-tRNA synthetase, Protein biosynthesis, Ligase, ATP-binding, Nucleotide-binding, Magnesium, Metal-binding.

GO:0005524; Molecular function: ATP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004826; Molecular function: phenylalanine-tRNA ligase activity.
GO:0006432; Biological process: phenylalanyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

From: SYFA_THETH (P27001)
Key		From		To		Description		Condition		FTGroup
METAL		262		262		Magnesium (By similarity)		E

Size range:	325-373 amino acids
Related UniRules:	MF_00282 (SYFA)
Template:	P08312 (SYFA_ECOLI); P27001 (SYFA_THETH): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	BUCAI and BUCAP lack the conserved Glu that bind magnesium