HAMAP rule MF_00283
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00283 |
| Accession | MF_00283 |
| Dates | 1-JUN-2001 (Created)
2-SEP-2024 (Last updated, Version 46) |
| Name | Phe_tRNA_synth_beta1 |
| Scope(s) |
Bacteria Plastid |
| Template(s) | P27002 (SYFB_THETH); [ Recover all ] |
| Triggered by |
HAMAP; MF_00283 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYFB |
| case not <OG:Chloroplast> | |
| Protein name | RecName: Full=Phenylalanine--tRNA ligase beta subunit; EC=6.1.1.20; AltName: Full=Phenylalanyl-tRNA synthetase beta subunit; Short=PheRS; |
| else case <OG:Chloroplast> | |
| Protein name | RecName: Full=Phenylalanine--tRNA ligase beta subunit, chloroplastic; EC=6.1.1.20; AltName: Full=Phenylalanyl-tRNA synthetase beta subunit; Short=PheRS; |
| end case | |
| Gene name | Name=pheT; |
Comments
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| CATALYTIC ACTIVITY | Reaction=tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+); Xref=Rhea:RHEA:19413, Rhea:RHEA-COMP:9668, Rhea:RHEA-COMP:9699, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58095, ChEBI:CHEBI:78442, ChEBI:CHEBI:78531, ChEBI:CHEBI:456215; EC=6.1.1.20; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 magnesium ions per tetramer.; |
| SUBUNIT | Tetramer of two alpha and two beta subunits. |
| case <OC:Bacteria> | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| else case <OG:Chloroplast> | |
| SUBCELLULAR LOCATION | Plastid, chloroplast. |
| end case | |
| SIMILARITY | Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. |
Keywords
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| case <OC:Bacteria> | |
| Cytoplasm | |
| end case | |
| Aminoacyl-tRNA synthetase | |
| Protein biosynthesis | |
| Ligase | |
| ATP-binding | |
| Nucleotide-binding | |
| Magnesium | |
| Metal-binding | |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding | |
| GO:0000287; Molecular function:magnesium ion binding | |
| GO:0004826; Molecular function:phenylalanine-tRNA ligase activity | |
| GO:0006432; Biological process:phenylalanyl-tRNA aminoacylation | |
| case <OCellular component:Bacteria> | |
| GO:0005737; Cellular component:cytoplasm | |
| else case <OG:Chloroplast> | |
| GO:0009507; Cellular component:chloroplast | |
| end case | |
Cross-references
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| Pfam | PF01588; tRNA_bind; 1; |
| NCBIfam | TIGR00471; PheT_arch; 1; |
| NCBIfam | TIGR00472; PheT_bact; 1; |
| PROSITE | PS51483; B5; 1; |
| PROSITE | PS50886; TRBD; 0-1; |
| PROSITE | PS51447; FDX_ACB; 0-1; |
Features
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| From: SYFB_THETH (P27002) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 452 | 452 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="shared with alpha subunit" |
D | ||||||||
| BINDING | 458 | 458 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="shared with alpha subunit" |
D | ||||||||
| BINDING | 461 | 461 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="shared with alpha subunit" |
E | ||||||||
| BINDING | 462 | 462 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="shared with alpha subunit" |
[ED] | ||||||||
Additional information
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| Size range | 706-869 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |