HAMAP

Accession	MF_00289
Dates	1-JUN-2001 (Created) 17-AUG-2010 (Last updated, Version 29)

Data class

Protein

Predictors

HAMAP; MF_00289_B; [distribution of match scores in UniProtKB];[seed alignment for MF_00289_B]

Predictors

HAMAP; MF_00289_A; [distribution of match scores in UniProtKB];[seed alignment for MF_00289_A]

Names

Proteasome_A

Identifier

PSA

Protein name

RecName: Full=Proteasome subunit alpha; EC=3.4.25.1; AltName: Full=20S proteasome alpha subunit; AltName: Full=Proteasome core protein PrcA;

Gene name

prcA

Protein name

RecName: Full=Proteasome subunit alpha; EC=3.4.25.1; AltName: Full=20S proteasome alpha subunit; AltName: Full=Proteasome core protein PsmA;

Gene name

psmA

FUNCTION: Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation (By similarity).

CATALYTIC ACTIVITY: Cleavage of peptide bonds with very broad specificity.

ENZYME REGULATION: The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity (By similarity).

PATHWAY: Protein degradation; proteasomal pup-dependent pathway.

SUBUNIT: The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC (By similarity).

ENZYME REGULATION: The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity (By similarity).

SUBUNIT: The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the peptidase T1A family.

PROSITE	PS00388; PROTEASOME_A_1; 1; PS51475; PROTEASOME_A_2; 1;
Pfam	PF00227; Proteasome; 1; PF10584; Proteasome_A_N; 1;
TIGRFAMs	TIGR03691; 20S_bact_alpha; 1; TIGR03633; Arc_protsome_A; 1;

Cytoplasm, Hydrolase, Protease, Proteasome, Threonine protease.

GO:0004298; Molecular function: threonine-type endopeptidase activity.
GO:0010498; Biological process: proteasomal protein catabolic process.

GO:0019941; Biological process: modification-dependent protein catabolic process.

GO:0005737; Cellular component: cytoplasm.
GO:0019773; Cellular component: proteasome core complex, alpha-subunit complex.

Size range:

230-292 amino acids

Size range:

233-261 amino acids

Related UniRules:	None
Template:	Q60177 (PSA_METJA); P25156 (PSA_THEAC); O33244 (PSA_MYCTU); Q9V2V6 (PSA1_HALVD); Q9V2V5 (PSA2_HALVD); Q53080 (PSA1_RHOER); Q53084 (PSA2_RHOER); O29760 (PSA_ARCFU): [Recover all]
Scope:	Archaea Bacteria; Actinobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in HALVD, RHOER
Plasmid encoded:	None
Comments:	Subunit beta of the proteasome core is in MF_02113.