To improve security and privacy, we are moving our web pages and services from HTTP to HTTPS.
To give users of web services time to transition to HTTPS, we will support separate HTTP and HTTPS services until the end of 2017.
From January 2018 most HTTP traffic will be automatically redirected to HTTPS. [more...]
View this page in https
Annotation rule MF_00295
Send feedback

General rule information [?]

Accession MF_00295
Dates 1-JUN-2001 (Created)
15-JUN-2017 (Last updated, Version 31)
Name MetA_acyltransf
Scope Bacteria
Templates Q72X44 (METAA_BACC1); P07623 (METAS_ECOLI); Q9WZY3 (METAA_THEMA); A0A1D3PCK2 (SAT_LACAI): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
Identifier
METAS
Protein name
RecName: Full=Homoserine O-succinyltransferase;
Short=HST;
EC=2.3.1.46;
AltName: Full=Homoserine transsuccinylase;
Short=HTS;
Gene name
metAS
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
Identifier
METAA
Protein name
RecName: Full=Homoserine O-acetyltransferase;
Short=HAT;
EC=2.3.1.31;
AltName: Full=Homoserine transacetylase;
Short=HTA;
Gene name
metAA
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
Identifier
SAT
Protein name
RecName: Full=Serine O-acetyltransferase;
Short=SAT;
EC=2.3.1.30;
else
Identifier
META
Protein name
RecName: Full=Probable acyltransferase;
EC=2.3.1.-;
end case

Comments [?]

case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
Function Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine.
Catalytic activity Succinyl-CoA + L-homoserine = CoA + O-succinyl-L-homoserine.
Pathway Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-succinyl-L-homoserine from L-homoserine: step 1/1.
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
Function Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine.
Catalytic activity Acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine.
Pathway Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
Function Transfers an acetyl group from acetyl-CoA to L-serine, forming acetyl-L-serine.
Catalytic activity Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.
Pathway Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.
end case
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Subunit Homodimer.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the MetA family.

Keywords [?]

case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
end case

Gene Ontology [?]

case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
GO:0004414; Molecular function: homoserine O-acetyltransferase activity.
GO:0009086; Biological process: methionine biosynthetic process.
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
GO:0008899; Molecular function: homoserine O-succinyltransferase activity.
GO:0009086; Biological process: methionine biosynthetic process.
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
GO:0009001; Molecular function: serine O-acetyltransferase activity.
GO:0006535; Biological process: cysteine biosynthetic process from serine.
end case
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF04204; HTS; 1;
PIRSF PIRSF000450; H_ser_succinyltr; 1;
TIGRFAMs TIGR01001; MetA; 1;

Features [?]

From: METAA_BACC1 (Q72X44)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     142     142       Acyl-thioester intermediate     C   1
ACT_SITE     235     235       Proton acceptor     H   1
ACT_SITE     237     237             E  
BINDING     163     163       Substrate     K   1
BINDING (Optional)     192     192       Substrate     S  
BINDING     249     249       Substrate     R   1
SITE (Optional)     109     109       Important for acyl-CoA specificity   P4   [NE]  
SITE (Optional)     111     111       Important for acyl-CoA specificity   P5a   [GA]  
SITE (Optional)     111     111       Important for acyl-CoA specificity   P5b   [ED]  
SITE (Optional)     143     143       Important for acyl-CoA specificity   P7   L  
SITE (Optional)     192     192       Important for substrate specificity   P10a   A  
SITE (Optional)     192     192       Important for substrate specificity   P10b   S  

Additional information [?]

Size range 250-385 amino acids
Related rules None
Fusion None
Comments Classification as HST, HAT or SAT was done according to Bastard et al. (2017) (PubMed:28581482).