HAMAP rule MF_00306
General rule information
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| Accession | MF_00306 |
| Dates | 1-JUN-2001 (Created)
2-SEP-2024 (Last updated, Version 36) |
| Name | SRP54 |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0AGD7 (SRP54_ECOLI); O29633 (SRP54_ARCFU); P37105 (SRP54_BACSU); O07347 (SRP54_THEAQ); Q977V2 (SRP54_HALVD); [ Recover all ] |
| Triggered by |
HAMAP; MF_00306 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SRP54 |
| case <OC:Archaea> | |
| Protein name | RecName: Full=Signal recognition particle 54 kDa protein; Short=SRP54; EC=3.6.5.4; |
| Gene name | Name=srp54; |
| else case <OC:Bacteria> | |
| Protein name | RecName: Full=Signal recognition particle protein; EC=3.6.5.4; AltName: Full=Fifty-four homolog; |
| Gene name | Name=ffh; |
| end case | |
Comments
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| case <OC:Archaea> | |
| FUNCTION | Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. |
| CATALYTIC ACTIVITY | Reaction=GTP + H2O = GDP + phosphate + H(+); Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.4; |
| SUBUNIT | Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19. |
| else case <OC:Pseudomonadota> | |
| FUNCTION | Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components. |
| CATALYTIC ACTIVITY | Reaction=GTP + H2O = GDP + phosphate + H(+); Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.4; |
| SUBUNIT | Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule. |
| else | |
| FUNCTION | Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. |
| CATALYTIC ACTIVITY | Reaction=GTP + H2O = GDP + phosphate + H(+); Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.4; |
| SUBUNIT | Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. Note=The SRP-RNC complex is targeted to the cytoplasmic membrane. |
| DOMAIN | Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC. |
| SIMILARITY | Belongs to the GTP-binding SRP family. SRP54 subfamily. |
Keywords
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| Cytoplasm |
| Signal recognition particle |
| GTP-binding |
| Hydrolase |
| Nucleotide-binding |
| Ribonucleoprotein |
| RNA-binding |
Gene Ontology
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| GO:0005525; Molecular function:GTP binding | |
| GO:0003924; Molecular function:GTPase activity | |
| case <OCellular component:Archaea> | |
| GO:0008312; Molecular function:7S RNA binding | |
| else case <OCellular component:Bacteria> | |
| GO:0003723; Molecular function:RNA binding | |
| end case | |
| GO:0006612; Biological process:protein targeting to membrane | |
| GO:0048500; Cellular component:signal recognition particle | |
| GO:1990904; Cellular component:ribonucleoprotein complex | |
Cross-references
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| Pfam | PF00448; SRP54; 1; |
| Pfam | PF02881; SRP54_N; 1; |
| Pfam | PF02978; SRP_SPB; 1; |
| PROSITE | PS00300; SRP54; 1; |
| NCBIfam | TIGR00959; Ffh; 1; |
Features
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| From: SRP54_METJA (Q57565) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 107 | 114 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
G-x-x-G-x(1,3)-G-K-T | ||||||||
| BINDING | 188 | 192 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
D-[ST]-[AS]-G-R | ||||||||
| BINDING | 247 | 250 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
x-[KR]-x-D | ||||||||
Additional information
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| Size range | 430-525 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |