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HAMAP rule MF_00315
General rule information
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| Accession | MF_00315 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 36) |
| Name | DXP_synth |
| Scope(s) |
Bacteria |
| Template(s) | P77488 (DXS_ECOLI); Q9RUB5 (DXS_DEIRA); [ Recover all ] |
| Triggered by |
HAMAP; MF_00315 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DXS |
| Protein name | RecName: Full=1-deoxy-D-xylulose-5-phosphate synthase; EC=2.2.1.7; AltName: Full=1-deoxyxylulose-5-phosphate synthase; Short=DXP synthase; Short=DXPS; |
| Gene name | Name=dxs; |
Comments
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| FUNCTION | Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D- xylulose-5-phosphate (DXP). |
| CATALYTIC ACTIVITY | Reaction=D-glyceraldehyde 3-phosphate + H(+) + pyruvate = 1-deoxy-D- xylulose 5-phosphate + CO2; Xref=Rhea:RHEA:12605, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57792, ChEBI:CHEBI:59776; EC=2.2.1.7; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| COFACTOR | Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Note=Binds 1 thiamine pyrophosphate per subunit.; |
| PATHWAY | Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5- phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D- glyceraldehyde 3-phosphate and pyruvate: step 1/1. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the transketolase family. DXPS subfamily. |
Keywords
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| Magnesium |
| Metal-binding |
| Transferase |
| Thiamine pyrophosphate |
| Isoprene biosynthesis |
| Thiamine biosynthesis |
Gene Ontology
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| GO:0000287; Molecular function:magnesium ion binding |
| GO:0008661; Molecular function:1-deoxy-D-xylulose-5-phosphate synthase activity |
| GO:0030976; Molecular function:thiamine pyrophosphate binding |
| GO:0016114; Biological process:terpenoid biosynthetic process |
| GO:0009228; Biological process:thiamine biosynthetic process |
Cross-references
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| NCBIfam | TIGR00204; Dxs; 1; |
| PROSITE | PS00801; TRANSKETOLASE_1; 1; |
| PROSITE | PS00802; TRANSKETOLASE_2; 1; |
Features
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| From: DXS_ECOLI (P77488) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 121 | 123 | /ligand="thiamine diphosphate" /ligand_id="ChEBI:CHEBI:58937" |
[GAS]-x-[SAT] | ||||||||
| BINDING | 153 | 154 | /ligand="thiamine diphosphate" /ligand_id="ChEBI:CHEBI:58937" |
[GA]-[ASTG] | ||||||||
| BINDING | 152 | 152 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 181 | 181 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
N | ||||||||
| BINDING | 80 | 80 | /ligand="thiamine diphosphate" /ligand_id="ChEBI:CHEBI:58937" |
H | ||||||||
| BINDING | 181 | 181 | /ligand="thiamine diphosphate" /ligand_id="ChEBI:CHEBI:58937" |
N | ||||||||
| BINDING | 288 | 288 | /ligand="thiamine diphosphate" /ligand_id="ChEBI:CHEBI:58937" |
[YFH] | ||||||||
| BINDING | 370 | 370 | /ligand="thiamine diphosphate" /ligand_id="ChEBI:CHEBI:58937" |
E | ||||||||
Additional information
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| Size range | 583-680 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Atypical sequences in BUCAP and LISMO with internal deletions not taken into account in size range and alignment |