HAMAP rule MF_00318
General rule information
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| Accession | MF_00318 |
| Dates | 1-JUN-2001 (Created)
2-FEB-2024 (Last updated, Version 65) |
| Name | Enolase |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0A6P9 (ENO_ECOLI); Q8GR70 (ENO_ENTHR); P37869 (ENO_BACSU); Q97QS2 (ENO_STRPN); P9WNL1 (ENO_MYCTU); [ Recover all ] |
| Triggered by |
HAMAP; MF_00318 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | ENO |
| Protein name | RecName: Full=Enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=2-phosphoglycerate dehydratase; |
| Gene name | Name=eno; |
Comments
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| FUNCTION | Catalyzes the reversible conversion of 2-phosphoglycerate (2- PG) into phosphoenolpyruvate (PEP). It is essential for the degradation of carbohydrates via glycolysis. |
| CATALYTIC ACTIVITY | Reaction=(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate; Xref=Rhea:RHEA:10164, ChEBI:CHEBI:15377, ChEBI:CHEBI:58289, ChEBI:CHEBI:58702; EC=4.2.1.11; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds a second Mg(2+) ion via substrate during catalysis.; |
| end case | |
| PATHWAY | Carbohydrate degradation; glycolysis; pyruvate from D- glyceraldehyde 3-phosphate: step 4/5. |
| case <OC:Gammaproteobacteria> | |
| SUBUNIT | Component of the RNA degradosome, a multiprotein complex involved in RNA processing and mRNA degradation. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. |
| SIMILARITY | Belongs to the enolase family. |
Keywords
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| Secreted | |
| Cytoplasm | |
| Lyase | |
| Glycolysis | |
| case <FTGroup:1> | |
| Magnesium | |
| Metal-binding | |
| end case | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
| GO:0004634; Molecular function:phosphopyruvate hydratase activity | |
| GO:0006096; Biological process:glycolytic process | |
| GO:0005737; Cellular component:cytoplasm | |
| GO:0009986; Cellular component:cell surface | |
Cross-references
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| Pfam | PF00113; Enolase_C; 1; |
| Pfam | PF03952; Enolase_N; 1; |
| PIRSF | PIRSF001400; Enolase; 1; |
| PRINTS | PR00148; ENOLASE; 1; |
| NCBIfam | TIGR01060; eno; 1; |
| PROSITE | PS00164; ENOLASE; 1; |
Features
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| From: ENO_ECOLI (P0A6P9) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 209 | 209 | /note="Proton donor" | [ED] | ||||||||
| ACT_SITE | 342 | 342 | /note="Proton acceptor" | K | ||||||||
| BINDING | 246 | 246 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 290 | 290 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | 1 | |||||||
| BINDING | 317 | 317 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 167 | 167 | /ligand="(2R)-2-phosphoglycerate" /ligand_id="ChEBI:CHEBI:58289" |
Q | ||||||||
| BINDING | 342 | 342 | /ligand="(2R)-2-phosphoglycerate" /ligand_id="ChEBI:CHEBI:58289" |
K | ||||||||
| BINDING | 371 | 371 | /ligand="(2R)-2-phosphoglycerate" /ligand_id="ChEBI:CHEBI:58289" |
R | ||||||||
| BINDING | 372 | 372 | /ligand_id="ChEBI:CHEBI:58289" /ligand="(2R)-2-phosphoglycerate" |
S | ||||||||
| BINDING | 393 | 393 | /ligand="(2R)-2-phosphoglycerate" /ligand_id="ChEBI:CHEBI:58289" |
K | ||||||||
Additional information
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| Size range | 369-550 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |