HAMAP rule MF_00318
General rule information
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Accession | MF_00318 |
Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 62) |
Name | Enolase |
Scope(s) |
Bacteria Archaea |
Template(s) | P0A6P9 (ENO_ECOLI); Q8GR70 (ENO_ENTHR); P37869 (ENO_BACSU); Q97QS2 (ENO_STRPN); [ Recover all ] |
Triggered by |
HAMAP; MF_00318 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | ENO |
Protein name | RecName: Full=Enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=2-phosphoglycerate dehydratase; |
Gene name | Name=eno; |
Comments
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FUNCTION | Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis. |
CATALYTIC ACTIVITY | Reaction=(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate; Xref=Rhea:RHEA:10164, ChEBI:CHEBI:15377, ChEBI:CHEBI:58289, ChEBI:CHEBI:58702; EC=4.2.1.11; |
case <FTGroup:1> | |
COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
end case | |
case <FTTag:inhib> | |
ACTIVITY REGULATION | The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein. |
end case | |
PATHWAY | Carbohydrate degradation; glycolysis; pyruvate from D- glyceraldehyde 3-phosphate: step 4/5. |
case <OC:Gammaproteobacteria> | |
SUBUNIT | Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. |
end case | |
SUBCELLULAR LOCATION | Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface. |
SIMILARITY | Belongs to the enolase family. |
Keywords
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Secreted | |
Cytoplasm | |
Lyase | |
Glycolysis | |
case <FTGroup:1> | |
Magnesium | |
Metal-binding | |
end case |
Gene Ontology
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case <FTGroup:1> | |
GO:0000287; Molecular function:magnesium ion binding | |
end case | |
GO:0004634; Molecular function:phosphopyruvate hydratase activity | |
GO:0006096; Biological process:glycolytic process | |
GO:0005737; Cellular component:cytoplasm | |
GO:0009986; Cellular component:cell surface |
Cross-references
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Pfam | PF00113; Enolase_C; 1; |
Pfam | PF03952; Enolase_N; 1; |
PIRSF | PIRSF001400; Enolase; 1; |
PRINTS | PR00148; ENOLASE; 1; |
NCBIfam | TIGR01060; Eno; 1; |
PROSITE | PS00164; ENOLASE; 1; |
Features
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From: ENO_ECOLI (P0A6P9) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 369 | 372 | /ligand="substrate" | S-H-R-S | ||||||||
ACT_SITE | 209 | 209 | /note="Proton donor" | [ED] | ||||||||
ACT_SITE | 342 | 342 | /note="Proton acceptor" | K | ||||||||
BINDING | 246 | 246 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
BINDING | 290 | 290 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | 1 | |||||||
BINDING | 317 | 317 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
BINDING | 159 | 159 | /ligand="substrate" | H | ||||||||
BINDING | 168 | 168 | /ligand="substrate" | [ED] | ||||||||
BINDING | 290 | 290 | /ligand="substrate" | E | ||||||||
BINDING | 317 | 317 | /ligand="substrate" | D | ||||||||
BINDING | 342 | 342 | /ligand="substrate" /note="covalent; in inhibited form" |
inhib | K | |||||||
BINDING | 393 | 393 | /ligand="substrate" | K |
Additional information
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Size range | 369-550 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |