HAMAP

Accession	MF_00318
Dates	1-JUN-2001 (Created) 9-JUN-2011 (Last updated, Version 49)

Data class

Protein

Predictors

HAMAP; MF_00318; [distribution of match scores in UniProtKB];[seed alignment for MF_00318]

Names

Enolase

Identifier

ENO

Protein name

RecName: Full=Enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=2-phosphoglycerate dehydratase;

Gene name

eno

FUNCTION: Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis. It is also a component of the RNA degradosome, a multi-enzyme complex involved in RNA processing and messenger RNA degradation. Its interaction with RNase E is important for the turnover of mRNA, in particular on transcripts encoding enzymes of energy-generating metabolic routes. Its presence in the degradosome is required for the response to excess phosphosugar. May play a regulatory role in the degradation of specific RNAs, such as ptsG mRNA, therefore linking cellular metabolic status with post-translational gene regulation (By similarity).

FUNCTION: Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis (By similarity).

CATALYTIC ACTIVITY: 2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O.

COFACTOR: Magnesium. Required for catalysis and for stabilizing the dimer (By similarity).

ENZYME REGULATION: The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein (By similarity).

PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

SUBUNIT: Homodimer. Interacts with the C-terminal region of the endoribonuclease RNase E in the RNA degradosome (By similarity).

SUBUNIT: Homooctamer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. As part of the bacterial cytoskeleton in the cytoplasm, is organized as extended coiled structures that wind around the cell, from one cell pole to the other. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial cell surface (By similarity).

SUBCELLULAR LOCATION: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface (By similarity).

SIMILARITY: Belongs to the enolase family.

Pfam	PF00113; Enolase_C; 1; PF03952; Enolase_N; 1;
PIRSF	PIRSF001400; Enolase; 1;
PRINTS	PR00148; ENOLASE; 1;
TIGRFAMs	TIGR01060; Eno; 1;
PROSITE	PS00164; ENOLASE; 1;

Acetylation.

Secreted, Cytoplasm.

Cytoskeleton.

Lyase, Glycolysis.

Magnesium, Metal-binding.

Phosphoprotein.

GO:0000287; Molecular function: magnesium ion binding.
GO:0004634; Molecular function: phosphopyruvate hydratase activity.
GO:0006096; Biological process: glycolysis.

GO:0005856; Cellular component: cytoskeleton.

GO:0005737; Cellular component: cytoplasm.
GO:0009986; Cellular component: cell surface.

From: ENO_ECOLI (P0A6P9)
Key		From		To		Description		Condition		FTGroup
REGION		369		372		Substrate binding (By similarity)		S-H-R-S
ACT_SITE		209		209		Proton donor (By similarity)		E
ACT_SITE		342		342		Proton acceptor (By similarity)		K
METAL		246		246		Magnesium (By similarity)		D		1
METAL		290		290		Magnesium (By similarity)		E		1
METAL		317		317		Magnesium (By similarity)		D		1
BINDING		159		159		Substrate (By similarity)		H
BINDING		168		168		Substrate (By similarity)		E
BINDING		290		290		Substrate (By similarity)		E
BINDING		317		317		Substrate (By similarity)		D
BINDING		342		342		Substrate (covalent); in inhibited form (By similarity)		K
BINDING		393		393		Substrate (By similarity)		K

Key		From		To		Description		Condition		FTGroup
MOD_RES		257		257		N6-acetyllysine (By similarity)		K

From: ENO_BACSU (P37869)
Key		From		To		Description		Condition		FTGroup
MOD_RES (Optional)		281		281		Phosphotyrosine (By similarity)		Y

Size range:	398-458 amino acids
Related UniRules:	None
Template:	P0A6P9 (ENO_ECOLI); Q8GR70 (ENO_ENTHR); P37869 (ENO_BACSU); Q97QS2 (ENO_STRPN): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	in CHLTE, DESHY, LACGA, LACJO, LACLA, LACLS, LACPL, METCA, PSESM, STRCO
Plasmid encoded:	in STRTR