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HAMAP rule MF_00333

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General rule information [?]

Accession MF_00333
Dates 1-JUN-2001 (Created)
17-FEB-2023 (Last updated, Version 45)
Name Coprogen_oxidas
Scope(s) Bacteria
Template(s) P36553 (HEM6_ECOLI); P72848 (HEM6_SYNY3); [ Recover all ]
Triggered by HAMAP; MF_00333 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier HEM6
Protein name RecName: Full=Oxygen-dependent coproporphyrinogen-III oxidase;
                 Short=CPO;
                 Short=Coprogen oxidase;
                 Short=Coproporphyrinogenase;
                 EC=1.3.3.3;
Gene name Name=hemF;

Comments [?]

case <OC:Cyanobacteriota>
FUNCTIONInvolved in the heme and chlorophyll biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.
else
FUNCTIONInvolved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen- IX.
end case
CATALYTIC ACTIVITY Reaction=coproporphyrinogen III + 2 H(+) + O2 = 2 CO2 + 2 H2O + protoporphyrinogen IX; Xref=Rhea:RHEA:18257, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:57307, ChEBI:CHEBI:57309; EC=1.3.3.3;
case <OC:Escherichia>
COFACTOR Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
else
COFACTOR Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
end case
PATHWAYPorphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.
SUBUNITHomodimer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the aerobic coproporphyrinogen-III oxidase family.

Keywords [?]

case <OC:Cyanobacteriota>
Chlorophyll biosynthesis
end case
Cytoplasm
Heme biosynthesis
case <OC:Escherichia>
Manganese
end case
Metal-binding
Oxidoreductase
Porphyrin biosynthesis

Gene Ontology [?]

GO:0004109; Molecular function:coproporphyrinogen oxidase activity
case <OCellular component:Escherichia>
GO:0030145; Molecular function:manganese ion binding
end case
GO:0042803; Molecular function:protein homodimerization activity
GO:0006779; Biological process:porphyrin-containing compound biosynthetic process
GO:0006783; Biological process:heme biosynthetic process
case <OCellular component:Cyanobacteriota>
GO:0015995; Biological process:chlorophyll biosynthetic process
end case
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01218; Coprogen_oxidas; 1;
PRINTS PR00073; COPRGNOXDASE; 1;
PROSITE PS01021; COPROGEN_OXIDASE; 1;

Features [?]

From: HEM6_ECOLI (P36553)
Key From To Description Tag Condition FTGroup
BINDING 108 110 /ligand="substrate" N-x-R
REGION 240 275 /note="Important for dimerization"
BINDING 258 260 /ligand="substrate" G-x-[NR]
ACT_SITE 106 106 /note="Proton donor" H
case <OC:Escherichia>
BINDING 96 96 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"		 H
BINDING 106 106 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"		 H
BINDING 145 145 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"		 H
BINDING 175 175 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"		 H
else
BINDING 96 96 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 H
BINDING 106 106 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 H
BINDING 145 145 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 H
BINDING 175 175 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 H
end case
BINDING 92 92 /ligand="substrate" S
SITE 175 175 /note="Important for dimerization" H

Additional information [?]

Size range 279-363 amino acids
Related rules None
Fusion Nter: None Cter: None



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