HAMAP

Accession	MF_00336
Dates	1-JUN-2001 (Created) 27-JUN-2011 (Last updated, Version 31)

Data class

Protein

Predictors

HAMAP; MF_00336; [distribution of match scores in UniProtKB];[seed alignment for MF_00336]

Names

BioD

Identifier

BIOD

Protein name

RecName: Full=ATP-dependent dethiobiotin synthetase BioD; EC=6.3.3.3; AltName: Full=DTB synthetase; Short=DTBS; AltName: Full=Dethiobiotin synthase;

Gene name

bioD

FUNCTION: Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring (By similarity).

CATALYTIC ACTIVITY: ATP + 7,8-diaminononanoate + CO(2) = ADP + phosphate + dethiobiotin.

COFACTOR: Magnesium (By similarity).

PATHWAY: Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.

SUBUNIT: Homodimer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm.

SIMILARITY: Belongs to the dethiobiotin synthetase family.

Pfam	PF01656; CbiA; 1;
PRINTS	PR00081; GDHRDH; 1;
TIGRFAMs	TIGR00347; BioD; 1;
PIRSF	PIRSF006755; DTB_synth; 1;

ATP-binding, Biotin biosynthesis, Cytoplasm, Ligase, Magnesium, Metal-binding, Nucleotide-binding.

GO:0005524; Molecular function: ATP binding.
GO:0004141; Molecular function: dethiobiotin synthase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0009102; Biological process: biotin biosynthetic process.

From: BIOD1_ECOLI (P13000)
Key		From		To		Description		Condition		FTGroup
NP_BIND		13		18		ATP (By similarity)		[EDNS]-[VAIL]-G-K-[TS]-[VLTI]
NP_BIND		116		119		ATP (By similarity)		E-X-X-G
NP_BIND		176		177		ATP (By similarity)		[NSM]-[DEHRQTNCSMK]
NP_BIND		205		207		ATP (By similarity)		P-X-[LIVSTG]
METAL		13		13		Magnesium 1 (By similarity)		[EDN]
METAL		17		17		Magnesium 2 (By similarity)		[TS]
METAL		55		55		Magnesium 2 (By similarity)		[DNCRHK]
METAL		116		116		Magnesium 2 (By similarity)		E
BINDING		42		42		Substrate (By similarity)		[ST]
BINDING		55		55		ATP (By similarity)		[DN]
BINDING		212		212		ATP (By similarity)		[ENQ]

Size range:	191-278 amino acids
Related UniRules:	None
Template:	P13000 (BIOD1_ECOLI); P22818 (BIOD_LYSSH): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: MF_00835 (BioC); Cter: None
Duplicate:	in ECO57, ECOLI, HAEDU, HAEIN, PASMU, SALTI, SALTY, YERPE
Plasmid encoded:	None