HAMAP rule MF_00336
General rule information
[?]
| Accession | MF_00336 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 43) |
| Name | BioD |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P13000 (BIOD1_ECOLI); P22818 (BIOD_LYSSH); P9WPQ5 (BIOD_MYCTU); O24872 (BIOD_HELPY); [ Recover all ] |
| Triggered by |
HAMAP; MF_00336 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | BIOD |
| Protein name | RecName: Full=ATP-dependent dethiobiotin synthetase BioD; EC=6.3.3.3; AltName: Full=DTB synthetase; Short=DTBS; AltName: Full=Dethiobiotin synthase; |
| Gene name | Name=bioD; |
Comments
[?]
| FUNCTION | Catalyzes a mechanistically unusual reaction, the ATP- dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8- diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring. |
| CATALYTIC ACTIVITY | Reaction=(7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)- dethiobiotin + ADP + 3 H(+) + phosphate; Xref=Rhea:RHEA:15805, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:149469, ChEBI:CHEBI:149473, ChEBI:CHEBI:456216; EC=6.3.3.3; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| PATHWAY | Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8- diaminononanoate: step 1/2. |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the dethiobiotin synthetase family. |
Keywords
[?]
Gene Ontology
[?]
| GO:0005524; Molecular function:ATP binding |
| GO:0004141; Molecular function:dethiobiotin synthase activity |
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0009102; Biological process:biotin biosynthetic process |
Cross-references
[?]
| NCBIfam | TIGR00347; BioD; 1; |
| PIRSF | PIRSF006755; DTB_synth; 1; |
Features
[?]
| From: BIOD1_ECOLI (P13000) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 13 | 18 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[EDNSG]-[VAILSC]-G-K-[TS]-[VLTIYFH] | ||||||||
| BINDING | 116 | 119 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
E-x-x-G | ||||||||
| BINDING | 176 | 177 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[NSMVILG]-[DEHRQTNCSMK] | ||||||||
| BINDING | 205 | 207 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[PA]-x-[LIVSTG] | ||||||||
| ACT_SITE | 38 | 38 | K | |||||||||
| BINDING | 17 | 17 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[TS] | ||||||||
| BINDING | 55 | 55 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[DNCRHK] | ||||||||
| BINDING | 116 | 116 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | ||||||||
| BINDING | 42 | 42 | /ligand="substrate" | [ST] | ||||||||
| BINDING | 55 | 55 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[DN] | ||||||||
| BINDING | 212 | 212 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[ENQ] | ||||||||
Additional information
[?]
| Size range | 191-278 amino acids |
| Related rules |
None |
| Fusion | Nter: MF_00835 (BioC) Cter: None |