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HAMAP rule MF_00418

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General rule information [?]

Accession MF_00418
Dates 19-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 46)
Name DapA
Scope(s) Bacteria
Archaea
Template(s) P0A6L2 (DAPA_ECOLI); P9WP25 (DAPA_MYCTU); Q9X1K9 (DAPA_THEMA); Q57695 (DAPA_METJA); Q6GH13 (DAPA_STAAR); Q5HG25 (DAPA_STAAC); Q9JZR4 (DAPA_NEIMB); Q9I4W3 (DAPA_PSEAE); Q07607 (DAPA_RHIML); Q8UGL3 (DAPA_AGRFC); [ Recover all ]
Triggered by HAMAP; MF_00418 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DAPA
Protein name RecName: Full=4-hydroxy-tetrahydrodipicolinate synthase;
                 Short=HTPA synthase;
                 EC=4.3.3.7;
Gene name Name=dapA;

Comments [?]

FUNCTIONCatalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).
CATALYTIC ACTIVITY Reaction=L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy- 2,3,4,5-tetrahydrodipicolinate + H(+) + H2O; Xref=Rhea:RHEA:34171, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:67139, ChEBI:CHEBI:537519; EC=4.3.3.7;
PATHWAYAmino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.
case (<OC:Bacteria> or <OC:Archaea>) and not (<OC:Staphylococcus> or <OC:Pseudomonas>)
SUBUNITHomotetramer; dimer of dimers.
else
SUBUNITHomodimer.
end case
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the DapA family.
CAUTIONWas originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)- dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.

Keywords [?]


Gene Ontology [?]

GO:0008840; Molecular function:4-hydroxy-tetrahydrodipicolinate synthase activity
GO:0019877; Biological process:diaminopimelate biosynthetic process
GO:0009085; Biological process:lysine biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00701; DHDPS; 1;
PRINTS PR00146; DHPICSNTHASE; 1;
NCBIfam TIGR00674; DapA; 1;
PROSITE PS00665; DHDPS_1; 1;
PROSITE PS00666; DHDPS_2; 1;

Features [?]

From: DAPA_ECOLI (P0A6L2)
Key From To Description Tag Condition FTGroup
ACT_SITE 133 133 /note="Proton donor/acceptor" Y
ACT_SITE 161 161 /note="Schiff-base intermediate with substrate" K
BINDING 45 45 /ligand="pyruvate"
/ligand_id="ChEBI:CHEBI:15361"
[TS]
BINDING 203 203 /ligand="pyruvate"
/ligand_id="ChEBI:CHEBI:15361"
[IVAT]
SITE 44 44 /note="Part of a proton relay during catalysis" [TS]
SITE 107 107 /note="Part of a proton relay during catalysis" Y

Additional information [?]

Size range 283-311 amino acids
Related rules MF_01237
MF_00694
Fusion Nter: None Cter: None



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