HAMAP rule MF_00421
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00421 |
| Accession | MF_00421 |
| Dates | 26-JUN-2001 (Created)
3-SEP-2024 (Last updated, Version 45) |
| Name | PurQ |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P12041 (PURQ_BACSU); Q9X0X2 (PURQ_THEMA); [ Recover all ] |
| Triggered by |
HAMAP; MF_00421 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PURQ |
| Protein name | RecName: Full=Phosphoribosylformylglycinamidine synthase subunit PurQ; Short=FGAM synthase; EC=6.3.5.3; AltName: Full=Formylglycinamide ribonucleotide amidotransferase subunit I; Short=FGAR amidotransferase I; Short=FGAR-AT I; AltName: Full=Phosphoribosylformylglycinamidine synthase subunit I; AltName: Full=Glutaminase PurQ; EC=3.5.1.2; |
| Gene name | Name=purQ; |
Comments
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| FUNCTION | Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP- dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL. |
| CATALYTIC ACTIVITY | Reaction=N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L- glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D- ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+); Xref=Rhea:RHEA:17129, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:147286, ChEBI:CHEBI:147287, ChEBI:CHEBI:456216; EC=6.3.5.3; |
| CATALYTIC ACTIVITY | Reaction=L-glutamine + H2O = L-glutamate + NH4(+); Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2; |
| PATHWAY | Purine metabolism; IMP biosynthesis via de novo pathway; 5- amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5- phospho-D-ribosyl)glycinamide: step 1/2. |
| SUBUNIT | Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits. |
| SUBCELLULAR LOCATION | Cytoplasm. |
Keywords
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| ATP-binding |
| Cytoplasm |
| Glutamine amidotransferase |
| Hydrolase |
| Ligase |
| Nucleotide-binding |
| Purine biosynthesis |
Gene Ontology
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| GO:0004642; Molecular function:phosphoribosylformylglycinamidine synthase activity |
| GO:0006189; Biological process:'de novo' IMP biosynthetic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: PURQ_THEMA (Q9X0X2) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 86 | 86 | /note="Nucleophile" | C | ||||||||
| ACT_SITE | 186 | 186 | H | |||||||||
| ACT_SITE | 188 | 188 | E | |||||||||
Additional information
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| Size range | 212-272 amino acids |
| Related rules |
MF_00419 |
| Fusion | Nter: None Cter: None |
| Comments | FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419), or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421) and PurS (MF_01926). |