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HAMAP annotation rule: MF_00443
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General rule information

Accession MF_00443
Dates 28-AUG-2001 (Created)
8-DEC-2011 (Last updated, Version 30)
Data class Protein
Predictors HAMAP; MF_00443; [distribution of match scores in UniProtKB];[seed alignment for MF_00443]
Names ThiG


Propagated annotation

Identifier, protein and gene names
Identifier THIG
Protein name
RecName: Full=Thiazole synthase;
EC=2.8.1.10;
Gene name thiG
Comments
FUNCTION: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S (By similarity).
CATALYTIC ACTIVITY: 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H(2)O.
PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis.
SUBUNIT: Homotetramer. Forms heterodimers with either ThiH or ThiS (By similarity).

case <OG:Chloroplast>
SUBCELLULAR LOCATION: Plastid, chloroplast.

else case not <OG:Chloroplast>
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
end case

SIMILARITY: Belongs to the ThiG family.
Cross-references
Pfam PF05690; ThiG; 1;
Keywords

case not <OG:Chloroplast>
Cytoplasm.
end case

Schiff base, Thiamine biosynthesis, Transferase.
Gene Ontology
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0009228; Biological process: thiamine biosynthetic process.

case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.

else case not <OG:Chloroplast>
GO:0005737; Cellular component: cytoplasm.
end case

Features
From: THIG_BACSU (O31618)
Key     From     To       Description   Condition   FTGroup
REGION     185     186       DXP binding (By similarity)   A-G  
REGION     207     208       DXP binding (By similarity)   x-[TS]  
ACT_SITE     98     98       Schiff-base intermediate with DXP (By similarity)   K  
BINDING     159     159       DXP; via amide nitrogen (By similarity)   G  


Additional information

Size range: 252-337 amino acids
Related UniRules: None
Template: O31618 (THIG_BACSU); P30139 (THIG_ECOLI): [Recover all]
Scope: Bacteria
Plastid
Fusion: Nter: <thiO>, <thiS>; Cter: None
Duplicate: in PELCD
Plasmid encoded: in ERWAM, ERWPY, RHIET, RHIME, STAS1
Comments: ThiO N-terminal domain (glycine oxidase) in NOSS1, ANAVT, and SYNY3. Possible wrong start in CYACA.

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UniProtKB rule member sequences