Annotation rule MF_00444
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General rule information [?]

Accession MF_00444
Dates 1-JUN-2001 (Created)
26-SEP-2014 (Last updated, Version 41)
Name ClpP
Scope Bacteria
Plastid
Template P0A6G7 (CLPP_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
CLPP
Protein name
RecName: Full=ATP-dependent Clp protease proteolytic subunit;
EC=3.4.21.92;
AltName: Full=Endopeptidase Clp;
Gene name
clpP

Comments [?]

Function Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.
Catalytic activity Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Subunit Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes. Component of the ClpAP and ClpXP complexes.
else case <OC:Bacteria>
Subunit Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.
else case <OC:Viridiplantae>
Subunit Component of the chloroplastic Clp protease core complex.
end case
case <OG:Chloroplast>
Subcellular location Plastid, chloroplast stroma.
end case
case not <OG:Chloroplast>
Subcellular location Cytoplasm.
end case
Similarity Belongs to the peptidase S14 family.

Keywords [?]

case not <OG:Chloroplast>
end case

Gene Ontology [?]

GO:0004252; Molecular function: serine-type endopeptidase activity.
GO:0006508; Biological process: proteolysis.
case <OG:Chloroplast>
GO:0009570; Cellular component: chloroplast stroma.
end case
case not <OG:Chloroplast>
GO:0005737; Cellular component: cytoplasm.
end case

Cross-references [?]

Pfam PF00574; CLP_protease; 1;
PRINTS PR00127; CLPPROTEASEP; 1;
TIGRFAMs TIGR00493; ClpP; 1;
PROSITE PS00381; CLP_PROTEASE_SER; 1;
PS00382; CLP_PROTEASE_HIS; 1;

Features [?]

From: CLPP_ECOLI (P0A6G7)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     111     111       Nucleophile     S  
ACT_SITE     136     136             H  

Additional information [?]

Size range 180-249 amino acids
Related rules None
Fusion None
Comments In Bacillus subtilis and also probably in some other Bacillaceae, ClpXP plays a major role in the third proteolytic step of stress-induced degradation of anti-sigma-W factor RsiW (PubMed=16899079). A number of possible inactive clpP-like protein have not been shown in the alignment (clpP4 from STRCO, clpR from SYNY3, etc.). Inteins deleted in CHLEU (not shown in alignment). Weird insert in CHLRE (not shown in alignment).