HAMAP

Accession	MF_00449
Dates	17-SEP-2001 (Created) 6-JUL-2011 (Last updated, Version 22)

Data class

Protein

Predictors

HAMAP; MF_00449; [distribution of match scores in UniProtKB];[seed alignment for MF_00449]

Names

RAD50

Identifier

RAD50

Protein name

RecName: Full=DNA double-strand break repair Rad50 ATPase;

Gene name

rad50

FUNCTION: Involved in DNA double-strand break repair (DSBR). The Rad50/Mre11 complex possesses single-strand endonuclease activity and ATP-dependent double-strand-specific 3'-5' exonuclease activity. Rad50 provides an ATP-dependent control of Mre11 by unwinding and/or repositioning DNA ends into the Mre11 active site (By similarity).

COFACTOR: Binds 1 zinc ion per heterotetramer (By similarity).

DOMAIN: The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer (By similarity).

SIMILARITY: Belongs to the SMC family. RAD50 subfamily.

PROSITE	PS51131; ZN_HOOK; 1; trigger=PRU00471;
Pfam	PF04423; Rad50_zn_hook; 1; PF02463; SMC_N; 1;
General	Coiled_coil; -; 0-unlimited; trigger=Yes;

DNA damage, DNA repair, Hydrolase, ATP-binding, Nucleotide-binding.

Metal-binding, Zinc.

GO:0005524; Molecular function: ATP binding.
GO:0016887; Molecular function: ATPase activity.
GO:0004519; Molecular function: endonuclease activity.
GO:0004527; Molecular function: exonuclease activity.
GO:0006302; Biological process: double-strand break repair.

GO:0008270; Molecular function: zinc ion binding.

From: RAD50_PYRFU (P58301)
Key		From		To		Description		Condition		FTGroup
NP_BIND		30		37		ATP (By similarity)		G-x(2)-[GA]-[SA]-G-K-[ST]
COMPBIAS		792		829		Ala/Asp-rich (DA-box)
METAL		444		444		Zinc (By similarity)		C		1
METAL		447		447		Zinc (By similarity)		C		1

Size range:	786-1074 amino acids
Related UniRules:	None
Template:	P58301 (RAD50_PYRFU)
Scope:	Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None