Annotation rule MF_00452
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General rule information [?]

Accession MF_00452
Dates 23-SEP-2001 (Created)
17-FEB-2014 (Last updated, Version 29)
Name PEPCK_GTP
Scope Bacteria
Archaea
Templates Q9AEM1 (PCKG_CORGL); Q9AGJ6 (PCKG_MYCSM); Q6F494 (PCKG_THEKO): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PCKG
Protein name
RecName: Full=Phosphoenolpyruvate carboxykinase [GTP];
Short=PEP carboxykinase;
Short=PEPCK;
EC=4.1.1.32;
Gene name
pckG

Comments [?]

Function Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (By similarity).
Catalytic activity GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO(2).
case <FTGroup:1>
Cofactor Binds 1 manganese ion per subunit (By similarity).
end case
case <OC:Bacteria>
Subunit Monomer (By similarity).
end case
Subcellular location Cytoplasm (By similarity).
Similarity Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

GO:0005525; Molecular function: GTP binding.
GO:0004613; Molecular function: phosphoenolpyruvate carboxykinase (GTP) activity.
case <FTGroup:1>
GO:0030145; Molecular function: manganese ion binding.
end case
GO:0006094; Biological process: gluconeogenesis.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00821; PEPCK; 1;
PROSITE PS00505; PEPCK_GTP; 1;

Features [?]

From: PCKG_CORGL (Q9AEM1)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND (Optional)     273     278       GTP (By similarity)     [AG]-x-G-K-T-N  
NP_BIND (Optional)     515     518       GTP (By similarity)     [FY]-x-x-N  
REGION     387     389       Substrate binding (By similarity)     N-x-R  
ACT_SITE     274     274       By similarity     [CS]  
METAL     230     230       Manganese (By similarity)     K   1
METAL     250     250       Manganese; via tele nitrogen (By similarity)     H   1
METAL     297     297       Manganese (By similarity)     D   1
BINDING     82     82       Substrate (By similarity)     R  
BINDING     223     223       Substrate; via amide nitrogen (By similarity)     G  
BINDING     230     230       Substrate (By similarity)     K  
BINDING     272     272       Substrate (By similarity)     S  
BINDING     389     389       GTP (By similarity)     R  
BINDING     420     420       GTP (By similarity)     R  

Additional information [?]

Size range 588-624 amino acids
Related rules None
Fusion None
Comments The enzyme from THEKO is shown to be a homotetramer.