HAMAP

Accession	MF_00452
Dates	23-SEP-2001 (Created) 13-DEC-2011 (Last updated, Version 26)

Data class

Protein

Predictors

HAMAP; MF_00452; [distribution of match scores in UniProtKB];[seed alignment for MF_00452]

Names

PEPCK_GTP

Identifier

PCKG

Protein name

RecName: Full=Phosphoenolpyruvate carboxykinase [GTP]; Short=PEP carboxykinase; Short=PEPCK; EC=4.1.1.32; AltName: Full=Phosphoenolpyruvate carboxylase;

Gene name

pckG

FUNCTION: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (By similarity).

CATALYTIC ACTIVITY: GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO(2).

COFACTOR: Binds 1 manganese ion per subunit (By similarity).

PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.

SUBUNIT: Monomer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Pfam	PF00821; PEPCK; 1;
PROSITE	PS00505; PEPCK_GTP; 1;

Cytoplasm, Decarboxylase, Gluconeogenesis, GTP-binding, Lyase.

Manganese, Metal-binding.

Nucleotide-binding.

GO:0005525; Molecular function: GTP binding.
GO:0004613; Molecular function: phosphoenolpyruvate carboxykinase (GTP) activity.

GO:0030145; Molecular function: manganese ion binding.

GO:0006094; Biological process: gluconeogenesis.
GO:0005737; Cellular component: cytoplasm.

From: PCKG_CORGL (Q9AEM1)
Key		From		To		Description		Condition		FTGroup
NP_BIND (Optional)		273		278		GTP (By similarity)		[AG]-x-G-K-T-N
NP_BIND (Optional)		515		518		GTP (By similarity)		[FY]-x-x-N
REGION		387		389		Substrate binding (By similarity)		N-x-R
ACT_SITE		274		274		By similarity		[CS]
METAL		230		230		Manganese (By similarity)		K		1
METAL		250		250		Manganese (By similarity)		H		1
METAL		297		297		Manganese (By similarity)		D		1
BINDING		82		82		Substrate (By similarity)		R
BINDING		223		223		Substrate; via amide nitrogen (By similarity)		G
BINDING		230		230		Substrate (By similarity)		K
BINDING		272		272		Substrate (By similarity)		S
BINDING		389		389		GTP (By similarity)		R
BINDING		420		420		GTP (By similarity)		R

Size range:	588-624 amino acids
Related UniRules:	None
Template:	Q9AEM1 (PCKG_CORGL); Q9AGJ6 (PCKG_MYCSM); Q6F494 (PCKG_PYRKO): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	The enzyme from PYRKO is shown to be a homotetramer.