HAMAP

Accession	MF_00497
Dates	4-DEC-2001 (Created) 17-AUG-2010 (Last updated, Version 23)

Data class

Protein

Predictors

HAMAP; MF_00497_B; [distribution of match scores in UniProtKB];[seed alignment for MF_00497_B]

Predictors

HAMAP; MF_00497_A; [distribution of match scores in UniProtKB];[seed alignment for MF_00497_A]

Names

G1P_dehydrogenase

Identifier

G1PDH

Protein name

RecName: Full=Glycerol-1-phosphate dehydrogenase [NAD(P)+]; Short=G1P dehydrogenase; Short=G1PDH; EC=1.1.1.261; AltName: Full=Enantiomeric glycerophosphate synthase; AltName: Full=sn-glycerol-1-phosphate dehydrogenase;

Gene name

egsA

FUNCTION: Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea (By similarity).

CATALYTIC ACTIVITY: sn-glycerol-1-phosphate + NAD(P)(+) = glycerone phosphate + NAD(P)H.

COFACTOR: Binds 1 zinc ion per subunit (By similarity).

PATHWAY: Membrane lipid metabolism; glycerophospholipid metabolism.

SUBUNIT: Homooctamer (By similarity).

SUBUNIT: Homodimer (By similarity).

FUNCTION: Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species (By similarity).

CATALYTIC ACTIVITY: sn-glycerol-1-phosphate + NAD(P)(+) = glycerone phosphate + NAD(P)H.

COFACTOR: Binds 1 nickel ion per subunit (By similarity).

SUBUNIT: Homodimer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (Potential).

SIMILARITY: Belongs to the glycerol-1-phosphate dehydrogenase family.

Pfam	PF01761; DHQ_synthase; 1;

Cytoplasm, Metal-binding, NAD, NADP.

Nickel.

Oxidoreductase, Phospholipid biosynthesis.

Zinc.

GO:0050492; Molecular function: glycerol-1-phosphate dehydrogenase [NAD(P)+] activity.
GO:0008654; Biological process: phospholipid biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

From: G1PDH_METTH (P72010)
Key		From		To		Description		Condition		FTGroup
NP_BIND		94		98		NAD (By similarity)		G-x-x-x-D
NP_BIND		116		119		NAD (By similarity)		T-x-x-S
METAL		168		168		Zinc; catalytic (By similarity)		[DE]
METAL		248		248		Zinc; catalytic (By similarity)		H
METAL		264		264		Zinc; catalytic (By similarity)		H
BINDING		121		121		Substrate (By similarity)		D
BINDING		125		125		NAD (By similarity)		S
BINDING		168		168		Substrate (By similarity)		[DE]
BINDING		252		252		Substrate (By similarity)		H

From: G1PDH_BACSU (P94527)
Key		From		To		Description		Condition		FTGroup
NP_BIND		116		120		NAD (By similarity)		G-x-x-x-D
NP_BIND		138		141		NAD (By similarity)		T-x-x-S
METAL		190		190		Nickel; catalytic (By similarity)		D
METAL		270		270		Nickel; catalytic (By similarity)		H
METAL		290		290		Nickel; catalytic (By similarity)		H
BINDING		54		54		NAD (By similarity)		D
BINDING		143		143		Substrate (By similarity)		D
BINDING		147		147		NAD (By similarity)		S
BINDING		190		190		Substrate (By similarity)		D
BINDING		274		274		Substrate (By similarity)		H

Size range:

394-404 amino acids

Size range:

314-360 amino acids

Related UniRules:	None
Template:	P72010 (G1PDH_METTH); Q9YER2 (G1PDH_AERPE); P94527 (G1PDH_BACSU): [Recover all]
Scope:	Bacteria; Bacillales Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None