HAMAP

Accession	MF_00523
Dates	13-JAN-2002 (Created) 4-NOV-2011 (Last updated, Version 27)

Data class

Protein

Predictors

HAMAP; MF_00523; [distribution of match scores in UniProtKB];[seed alignment for MF_00523]

Names

LpxD

Identifier

LPXD

Protein name

RecName: Full=UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase; Short=UDP-3-O-(3-OHC14)-GlcN N-acyltransferase; EC=2.3.1.191;

Protein name

RecName: Full=UDP-3-O-acylglucosamine N-acyltransferase; EC=2.3.1.-;

Gene name

lpxD

FUNCTION: Catalyzes the N-acylation of UDP-3-O-(hydroxymyristoyl)glucosamine using 3-hydroxymyristoyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell (By similarity).

CATALYTIC ACTIVITY: (3R)-3-hydroxymyristoyl-[acyl-carrier-protein] + UDP-3-O-((3R)-hydroxymyristoyl)-alpha-D-glucosamine = UDP-2,3-bis(O-(3R)-3-hydroxymyristoyl)-alpha-D-glucosamine + a holo-[acyl-carrier-protein].

PATHWAY: Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 3/6.

FUNCTION: Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell (By similarity).

CATALYTIC ACTIVITY: (3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein].

PATHWAY: Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.

SUBUNIT: Homotrimer (By similarity).

SIMILARITY: Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Pfam	PF00132; Hexapep; 6-9; PF04613; LpxD; 1;
TIGRFAMs	TIGR01853; Lipid_A_lpxD; 1;
PROSITE	PS00101; HEXAPEP_TRANSFERASES; 1-4;

Transferase, Acyltransferase, Lipid A biosynthesis, Lipid synthesis, Repeat.

GO:0016747; Molecular function: transferase activity, transferring acyl groups other than amino-acyl groups.
GO:0009245; Biological process: lipid A biosynthetic process.

From: LPXD_ECOLI (P21645)
Key		From		To		Description		Condition		FTGroup
ACT_SITE		239		239		Proton acceptor (By similarity)		H

Size range:	316-380 amino acids
Related UniRules:	MF_00387 (LPXA)
Template:	P21645 (LPXD_ECOLI); P0CD76 (LPXD_CHLTR): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in KORVE, FRAT1, FRATH, FRATO, FRATT, GLOVI, LEGPA, LEGPH, LEGPL, NITWN, RHOPB, SULDN
Plasmid encoded:	None
Comments:	The acyl chain added by LpxD varies depending on the species since there are differences in the structure of lipid A between different species. See Figure 9 from PubMed=19655786, the acyl chain length can vary from C10 to C20. The acyl chain present in the UDP-glucosamine substrate also seems to vary depending on the species (it is UDP-3-O-(hydroxymyristoyl)glucosamine in E.coli and UDP-3-O-myristoylglucosamine in Chlamydia trachomatis).