HAMAP

Accession	MF_00537
Dates	24-NOV-2006 (Created) 4-OCT-2010 (Last updated, Version 9)

Data class

Protein

Predictors

HAMAP; MF_00537; [distribution of match scores in UniProtKB];[seed alignment for MF_00537]

Names

Ribosomal_S14_1

Identifier

RS14

Protein name

RecName: Full=30S ribosomal protein S14;

Gene name

rpsN

Identifier

RS14

Protein name

RecName: Full=30S ribosomal protein S14;

Gene name

rpsN, rps14

Identifier

RR14

Protein name

RecName: Full=30S ribosomal protein S14, chloroplastic;

Gene name

rps14

FUNCTION: Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site (By similarity).

SUBUNIT: Part of the 30S ribosomal subunit. Contacts proteins S3 and S10 (By similarity).

FUNCTION: Binds 16S rRNA, required for the assembly of 30S particles (By similarity).

SUBUNIT: Part of the 30S ribosomal subunit (By similarity).

SUBCELLULAR LOCATION: Plastid, chloroplast.

SIMILARITY: Belongs to the ribosomal protein S14P family.

PROSITE	PS00527; RIBOSOMAL_S14; 1;
Pfam	PF00253; Ribosomal_S14; 1;

Ribonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding.

GO:0006412; Biological process: translation.
GO:0019843; Molecular function: rRNA binding.

GO:0009507; Cellular component: chloroplast.

Size range:	89-103 amino acids
Related UniRules:	MF_01364 (RS14Z)
Template:	P0AG59 (RS14_ECOLI); Q5SHQ1 (RS14Z_THET8): [Recover all]
Scope:	Bacteria Plastid
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	in LACS1
Comments:	In bacteria this gene has been subject to horizontal gene transfer, subsequent differential gene loss and insertions/deletions near the N-terminus. The ancestral version is most likely the shortest one (group I of PubMed 11102698) which has conserved cysteine residues that in the case of Thermus thermophilus have been shown to bind zinc. We have chosen to use the identifier RS14Z in bacteria for the zinc-binding subfamily (MF_01364). The group II proteins have an approximately 23 amino acid insertion and the group III proteins have an approximately 38 residue insertion; we do not distinguish between them however (this family, MF_00537); members of this subfamily do not usually possess the conserved cysteine residues. Many bacteria possess more than one copy of RS14, usually one of which is able to bind zinc. Interestingly, in the proteobacteria (which usually have the group III form) the acquired form is found within the spc operon, signifying gene replacement took place within the operon. The proteins in Legionella species and SALRD match this family but have the conserved residues necessary to bind zinc; whether they do so is unknown. See: PubMed=11102698; Brochier C., Philippe H., Moreira D.; "The evolutionary history of ribosomal protein RpS14: horizontal gene transfer at the heart of the ribosome."; Trends Genet. 16:529-533(2000). PubMed=11574053; Makarova K.S., Ponomarev V.A., Koonin E.V.; "Two C or not two C: recurrent disruption of Zn-ribbons, gene duplication, lineage-specific gene loss, and horizontal gene transfer in evolution of bacterial ribosomal proteins."; Genome Biol. 2:RESEARCH 0033-RESEARCH 0033(2001).