HAMAP

Accession	MF_00553
Dates	9-MAY-2002 (Created) 17-AUG-2010 (Last updated, Version 24)

Data class

Protein

Predictors

HAMAP; MF_00553; [distribution of match scores in UniProtKB];[seed alignment for MF_00553]

Names

PAN

Identifier

PAN

Protein name

RecName: Full=Proteasome-activating nucleotidase; Short=PAN; AltName: Full=Proteasomal ATPase; AltName: Full=Proteasome regulatory ATPase; AltName: Full=Proteasome regulatory particle;

Gene name

pan

FUNCTION: ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates (By similarity).

SUBUNIT: Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

DOMAIN: Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type (By similarity).

SIMILARITY: Belongs to the AAA ATPase family.

PROSITE	PS00674; AAA; 1;
Pfam	PF00004; AAA; 1;
TIGRFAMs	TIGR01242; 26Sp45; 1;
General	Coiled_coil; -; 0-unlimited; trigger=Yes;

Chaperone, Cytoplasm, Proteasome, ATP-binding, Nucleotide-binding.

GO:0005524; Molecular function: ATP binding.
GO:0016887; Molecular function: ATPase activity.
GO:0010498; Biological process: proteasomal protein catabolic process.
GO:0043335; Biological process: protein unfolding.
GO:0000502; Cellular component: proteasome complex.
GO:0005737; Cellular component: cytoplasm.
GO:0022623; Cellular component: proteasome-activating nucleotidase complex.

From: PAN_METJA (Q58576)
Key		From		To		Description		Condition		FTGroup
NP_BIND		214		219		ATP (By similarity)		G-x-G-K-T-[LM]
REGION		428		430		Docks into pockets in the proteasome alpha-ring to cause gate opening (By similarity)		x-[YF]-x
BINDING		353		353		ATP (By similarity)		[HY]

Size range:	392-436 amino acids
Related UniRules:	None
Template:	Q58576 (PAN_METJA); O28303 (PAN_ARCFU); D4GUJ7 (PAN1_HALVD); Q5UT56 (PAN2_HALVD): [Recover all]
Scope:	Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	in HALSA, HALVD
Plasmid encoded:	None
Comments:	The protein performing the same function in bacteria (called ARC) is in MF_02112.