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HAMAP rule MF_00563
General rule information
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Accession | MF_00563 |
Dates | 4-JUN-2002 (Created)
15-FEB-2024 (Last updated, Version 42) |
Name | AdoHcyase |
Scope(s) |
Bacteria Archaea |
Template(s) | P10760 (SAHH_RAT); P28183 (SAHH_RHOCB); Q58783 (SIHH_METJA); Q3JY79 (SAHH_BURP1); [ Recover all ] |
Triggered by |
HAMAP; MF_00563 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
Identifier | SIHH |
Protein name | RecName: Full=S-inosyl-L-homocysteine hydrolase; Short=SIHH; EC=3.13.1.9; |
else | |
Identifier | SAHH |
Protein name | RecName: Full=Adenosylhomocysteinase; EC=3.13.2.1; AltName: Full=S-adenosyl-L-homocysteine hydrolase; Short=AdoHcyase; |
Gene name | Name=ahcY; |
end case |
Comments
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case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
FUNCTION | Catalyzes the hydrolysis of S-inosyl-L-homocysteine (SIH) to L-homocysteine (Hcy) and inosine. Likely functions in a S-adenosyl-L- methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. Can also catalyze the reverse reaction in vitro, i.e. the synthesis of SIH from Hcy and inosine. |
CATALYTIC ACTIVITY | Reaction=H2O + S-inosyl-L-homocysteine = inosine + L-homocysteine; Xref=Rhea:RHEA:59828, ChEBI:CHEBI:15377, ChEBI:CHEBI:17596, ChEBI:CHEBI:57985, ChEBI:CHEBI:58199; EC=3.13.1.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59829; |
PATHWAY | Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis. |
MISCELLANEOUS | SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L- homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine. |
else | |
FUNCTION | May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine. |
CATALYTIC ACTIVITY | Reaction=H2O + S-adenosyl-L-homocysteine = adenosine + L-homocysteine; Xref=Rhea:RHEA:21708, ChEBI:CHEBI:15377, ChEBI:CHEBI:16335, ChEBI:CHEBI:57856, ChEBI:CHEBI:58199; EC=3.13.2.1; |
PATHWAY | Amino-acid biosynthesis; L-homocysteine biosynthesis; L- homocysteine from S-adenosyl-L-homocysteine: step 1/1. |
end case | |
COFACTOR | Name=NAD(+); Xref=ChEBI:CHEBI:57540; Note=Binds 1 NAD(+) per subunit.; |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | Belongs to the adenosylhomocysteinase family. |
Keywords
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Gene Ontology
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case <OCellular component:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
GO:0016802; Molecular function:trialkylsulfonium hydrolase activity | |
GO:0006556; Biological process:S-adenosylmethionine biosynthetic process | |
else; https://www.ebi.ac.uk/QuickGO/term/else | |
GO:0004013; Molecular function:adenosylhomocysteinase activity | |
GO:0071269; Biological process:L-homocysteine biosynthetic process | |
end case | |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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Pfam | PF05221; AdoHcyase; 1; |
Pfam | PF00670; AdoHcyase_NAD; 1; |
PIRSF | PIRSF001109; Ad_hcy_hydrolase; 1; |
NCBIfam | TIGR00936; AhcY; 1; |
PROSITE | PS00738; ADOHCYASE_1; 1; |
PROSITE | PS00739; ADOHCYASE_2; 1; |
Features
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From: SAHH_BURP1 (Q3JY79) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 200 | 202 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
T-T-[TA] | ||||||||
BINDING | 263 | 268 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
G-[YF]-G-x-x-G | ||||||||
BINDING | 342 | 344 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
[ISA]-G-H | ||||||||
BINDING | 64 | 64 | /ligand="substrate" | T | ||||||||
BINDING | 139 | 139 | /ligand="substrate" | D | ||||||||
BINDING | 199 | 199 | /ligand="substrate" | E | ||||||||
BINDING | 229 | 229 | /ligand="substrate" | K | ||||||||
BINDING | 233 | 233 | /ligand="substrate" | D | ||||||||
BINDING | 234 | 234 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
N | ||||||||
BINDING | 286 | 286 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
E | ||||||||
BINDING | 321 | 321 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
N | ||||||||
BINDING | 387 | 387 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
N |
Additional information
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Size range | 404-496 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |