HAMAP rule MF_00572
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00572 |
| Accession | MF_00572 |
| Dates | 12-JUL-2002 (Created)
25-APR-2024 (Last updated, Version 34) |
| Name | LeuA_type2 |
| Scope(s) |
Bacteria |
| Template(s) | P42455 (LEU1_CORGL); P9WQB3 (LEU1_MYCTU); [ Recover all ] |
| Triggered by |
HAMAP; MF_00572 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | LEU1 |
| Protein name | RecName: Full=2-isopropylmalate synthase; EC=2.3.3.13; AltName: Full=Alpha-IPM synthase; AltName: Full=Alpha-isopropylmalate synthase; |
| Gene name | Name=leuA; |
Comments
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| FUNCTION | Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate). |
| CATALYTIC ACTIVITY | Reaction=3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2- isopropylmalate + CoA + H(+); Xref=Rhea:RHEA:21524, ChEBI:CHEBI:1178, ChEBI:CHEBI:11851, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.13; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| end case | |
| PATHWAY | Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. |
Keywords
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| Amino-acid biosynthesis | |
| Branched-chain amino acid biosynthesis | |
| Cytoplasm | |
| Leucine biosynthesis | |
| Transferase | |
| case <FTGroup:1> | |
| Magnesium | |
| Metal-binding | |
| end case | |
Gene Ontology
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| GO:0005737; Cellular component:cytoplasm | |
| GO:0003852; Molecular function:2-isopropylmalate synthase activity | |
| GO:0003985; Molecular function:acetyl-CoA C-acetyltransferase activity | |
| GO:0009098; Biological process:L-leucine biosynthetic process | |
| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
Cross-references
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| Pfam | PF00682; HMGL-like; 1; |
| Pfam | PF08502; LeuA_dimer; 1; |
| NCBIfam | TIGR00970; leuA_yeast; 1; |
| PROSITE | PS00815; AIPM_HOMOCIT_SYNTH_1; 1; |
| PROSITE | PS00816; AIPM_HOMOCIT_SYNTH_2; 1; |
| PROSITE | PS50991; PYR_CT; 1; |
Features
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| From: LEU1_MYCTU (P9WQB3) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 491 | Cter | /note="Regulatory domain" | |||||||||
| BINDING | 81 | 81 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 285 | 285 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
H | 1 | |||||||
| BINDING | 287 | 287 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
H | 1 | |||||||
| BINDING | 321 | 321 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
N | 1 | |||||||
Additional information
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| Size range | 530-675 amino acids |
| Related rules |
MF_01025 MF_01028 |
| Fusion | Nter: None Cter: None |
| Comments | Some Mycobacteria have variable number tandem repeats (VNTR) insertions in this gene; for example M.tuberculosis strains H37Rv and CDC 1551 have 2 repeats, M.bovis strain ATCC BAA-935 / AF2122/97 has 5 repeats. |