HAMAP

Accession	MF_00614
Dates	31-JAN-2003 (Created) 2-DEC-2010 (Last updated, Version 16)

Data class

Protein

Predictors

HAMAP; MF_00614; [distribution of match scores in UniProtKB];[seed alignment for MF_00614]

Names

Fen

Identifier

FEN

Protein name

RecName: Full=Flap endonuclease 1; Short=FEN-1; EC=3.1.-.-; AltName: Full=Flap structure-specific endonuclease 1;

Gene name

fen

FUNCTION: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity).

COFACTOR: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding (By similarity).

SUBUNIT: Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity (By similarity).

SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Pfam	PF00867; XPG_I; 1; PF00752; XPG_N; 1;
PRINTS	PR00853; XPGRADSUPER; 1;
TIGRFAMs	TIGR03674; Fen_arch; 1;
PROSITE	PS00841; XPG_1; 1;

DNA damage, DNA repair, DNA replication, Endonuclease, Exonuclease, Hydrolase, Magnesium, Metal-binding, Nuclease.

GO:0003677; Molecular function: DNA binding.
GO:0008409; Molecular function: 5'-3' exonuclease activity.
GO:0017108; Molecular function: 5'-flap endonuclease activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0043137; Biological process: DNA replication, removal of RNA primer.
GO:0006281; Biological process: DNA repair.

From: FEN_METJA (Q58839)
Key		From		To		Description		Condition		FTGroup
REGION		1		98		N-domain
REGION		116		245		I-domain
REGION (Optional)		317		325		Interaction with PCNA (By similarity)		x-Q-x(2)-[ILM]-x(2)-[FYW]-[FYILAT]
METAL		27		27		Magnesium 1 (By similarity)		D
METAL		80		80		Magnesium 1 (By similarity)		D
METAL		152		152		Magnesium 1 (By similarity)		E
METAL		154		154		Magnesium 1 (By similarity)		E
METAL		173		173		Magnesium 2 (By similarity)		D
METAL		175		175		Magnesium 2 (By similarity)		D
METAL		224		224		Magnesium 2 (By similarity)		D

Size range:	326-351 amino acids
Related UniRules:	None
Template:	Q58839 (FEN_METJA); O93634 (FEN_PYRFU): [Recover all]
Scope:	Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None