Annotation rule MF_00641
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General rule information [?]

Accession MF_00641
Dates 26-APR-2003 (Created)
20-MAR-2014 (Last updated, Version 22)
Name Malate_synth_G
Scope Bacteria
Templates P42450 (MASZ_CORGL); P37330 (MASZ_ECOLI); P9WK17 (MASZ_MYCTU): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
MASZ
Protein name
RecName: Full=Malate synthase G;
EC=2.3.3.9;
Gene name
glcB

Comments [?]

Function Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA (By similarity).
Catalytic activity Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA.
Cofactor Magnesium (By similarity).
Pathway Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate; step 2/2.
Subunit Monomer (By similarity).
Subcellular location Cytoplasm (By similarity).
Similarity Belongs to the malate synthase family. GlcB subfamily.

Keywords [?]


Gene Ontology [?]

GO:0004474; Molecular function: malate synthase activity.
GO:0006097; Biological process: glyoxylate cycle.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01274; Malate_synthase; 1;
TIGRFAMs TIGR01345; Malate_syn_G; 1;

Features [?]

From: MASZ_ECOLI (P37330)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     125     126       Acetyl-CoA binding (By similarity)        
REGION     452     455       Glyoxylate binding (By similarity)        
ACT_SITE     338     338       Proton acceptor (By similarity)     R  
ACT_SITE     631     631       Proton donor (By similarity)     D  
METAL     427     427       Magnesium (By similarity)     E  
METAL     455     455       Magnesium (By similarity)     D  
BINDING     118     118       Acetyl-CoA; via carbonyl oxygen (By similarity)     V  
BINDING     274     274       Acetyl-CoA (By similarity)     S  
BINDING     311     311       Acetyl-CoA (By similarity)     R  
BINDING     338     338       Glyoxylate (By similarity)     R  
BINDING     427     427       Glyoxylate (By similarity)     E  
BINDING     536     536       Acetyl-CoA; via carbonyl oxygen (By similarity)     P  
MOD_RES     617     617       Cysteine sulfenic acid (-SOH) (By similarity)     C  
MOD_RES     688     688       Cysteine sulfenic acid (-SOH) (By similarity)     C  

Additional information [?]

Size range 721-748 amino acids
Related rules None
Fusion None