HAMAP rule MF_00716
General rule information
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Accession | MF_00716 |
Dates | 2-OCT-2003 (Created)
28-NOV-2023 (Last updated, Version 41) |
Name | NosZ |
Scope(s) |
Bacteria |
Template(s) | Q51705 (NOSZ_PARDE); P19573 (NOSZ_STUST); [ Recover all ] |
Triggered by |
HAMAP; MF_00716 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | NOSZ |
Protein name | RecName: Full=Nitrous-oxide reductase; EC=1.7.2.4; AltName: Full=N(2)OR; AltName: Full=N2O reductase; Flags: Precursor; |
Gene name | Name=nosZ; |
Comments
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FUNCTION | Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide. |
CATALYTIC ACTIVITY | Reaction=2 Fe(III)-[cytochrome c] + H2O + N2 = 2 Fe(II)-[cytochrome c] + 2 H(+) + nitrous oxide; Xref=Rhea:RHEA:43108, Rhea:RHEA-COMP:10350, Rhea:RHEA-COMP:14399, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17045, ChEBI:CHEBI:17997, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034; EC=1.7.2.4; |
COFACTOR | Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 2 calcium ions per subunit.; |
COFACTOR | Name=Cu cation; Xref=ChEBI:CHEBI:23378; Note=Binds 6 Cu cations per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction.; |
SUBUNIT | Homodimer. |
SUBCELLULAR LOCATION | Periplasm. |
case <Feature:PS51318> | |
PTM | Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. |
end case | |
SIMILARITY | Belongs to the NosZ family. |
SIMILARITY | In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family. |
Keywords
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Gene Ontology
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GO:0005509; Molecular function:calcium ion binding |
GO:0005507; Molecular function:copper ion binding |
GO:0050304; Molecular function:nitrous-oxide reductase activity |
GO:0042597; Cellular component:periplasmic space |
Cross-references
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Pfam | PF00394; Cu-oxidase; 1; |
NCBIfam | TIGR04244; nitrous_NosZ_RR; 1; |
NCBIfam | TIGR01409; TAT_signal_seq; 1; |
PROSITE | PS00078; COX2; 1; |
PROSITE | PS51318; TAT; 0-1; |
General | Signal; -; 1; |
Features
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From: NOSZ_PARDE (Q51705) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 554 | Cter | /note="COX2-like" | |||||||||
BINDING | 145 | 145 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="Z2" |
H | 1 | |||||||
BINDING | 146 | 146 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="Z3" |
H | 1 | |||||||
BINDING | 194 | 194 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="Z2" |
H | 1 | |||||||
BINDING | 271 | 271 | /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="2" |
Y | 3 | |||||||
BINDING | 274 | 274 | /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="2" |
E | 3 | |||||||
BINDING | 282 | 282 | /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="2" |
M | 3 | |||||||
BINDING | 288 | 288 | /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="2" |
D | 3 | |||||||
BINDING | 335 | 335 | /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="2" |
[NS] | 3 | |||||||
BINDING | 337 | 337 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="Z1" |
H | 1 | |||||||
BINDING | 392 | 392 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="Z1" |
H | 1 | |||||||
BINDING | 443 | 443 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="Z3" |
H | 1 | |||||||
BINDING | 464 | 464 | /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="1" |
K | 3 | |||||||
BINDING | 479 | 479 | /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="1" |
E | 3 | |||||||
BINDING | 504 | 504 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="Z4" |
H | 1 | |||||||
BINDING | 595 | 595 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="A1" |
H | 2 | |||||||
BINDING | 630 | 630 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="A1" |
C | 2 | |||||||
BINDING | 630 | 630 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="A2" |
C | 2 | |||||||
BINDING | 632 | 632 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="A2" |
[WH] | 2 | |||||||
BINDING | 634 | 634 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="A1" |
C | 2 | |||||||
BINDING | 634 | 634 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="A2" |
C | 2 | |||||||
BINDING | 638 | 638 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="A2" |
H | 2 | |||||||
BINDING | 641 | 641 | /ligand="Cu cation" /ligand_id="ChEBI:CHEBI:23378" /ligand_label="A1" |
M | 2 |
Additional information
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Size range | 634-652 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |