HAMAP

Accession	MF_00716
Dates	2-OCT-2003 (Created) 10-JUN-2011 (Last updated, Version 28)

Data class

Protein

Predictors

HAMAP; MF_00716; [distribution of match scores in UniProtKB];[seed alignment for MF_00716]

Names

NosZ

Identifier

NOSZ

Protein name

RecName: Full=Nitrous-oxide reductase; EC=1.7.2.4; AltName: Full=N(2)OR; AltName: Full=N2O reductase; Flags: Precursor;

Gene name

nosZ

FUNCTION: Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide (By similarity).

CATALYTIC ACTIVITY: Nitrogen + H(2)O + 2 cytochrome c = nitrous oxide + 2 reduced cytochrome c.

COFACTOR: Binds 2 calcium ions per subunit (By similarity).

COFACTOR: Binds 6 copper ions per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction (By similarity).

SUBUNIT: Homodimer (By similarity).

SUBCELLULAR LOCATION: Periplasm (By similarity).

PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

SIMILARITY: Belongs to the nosZ family.

SIMILARITY: In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

Pfam	PF00394; Cu-oxidase; 1;
TIGRFAMs	TIGR01409; TAT_signal_seq; 1;
PROSITE	PS00078; COX2; 1; PS51318; TAT; 0-1; trigger=PRU00648;

General

Signal; -; 1; trigger=Yes;

Oxidoreductase, Periplasm, Metal-binding, Calcium, Copper, Signal.

GO:0005509; Molecular function: calcium ion binding.
GO:0005507; Molecular function: copper ion binding.
GO:0050304; Molecular function: nitrous-oxide reductase activity.
GO:0042597; Cellular component: periplasmic space.

From: NOSZ_PARDE (Q51705)
Key		From		To		Description		Condition		FTGroup
REGION		554		Cter		COX2-like
METAL		145		145		Copper Z2 (By similarity)		H		1
METAL		146		146		Copper Z3 (By similarity)		H		1
METAL		194		194		Copper Z2 (By similarity)		H		1
METAL		271		271		Calcium 2; via carbonyl oxygen (By similarity)		Y		3
METAL		274		274		Calcium 2 (By similarity)		E		3
METAL		282		282		Calcium 2; via carbonyl oxygen (By similarity)		M		3
METAL		288		288		Calcium 2 (By similarity)		D		3
METAL		335		335		Calcium 2 (By similarity)		[NS]		3
METAL		337		337		Copper Z1 (By similarity)		H		1
METAL		392		392		Copper Z1 (By similarity)		H		1
METAL		443		443		Copper Z3 (By similarity)		H		1
METAL		464		464		Calcium 1; via carbonyl oxygen (By similarity)		K		3
METAL		479		479		Calcium 1 (By similarity)		E		3
METAL		504		504		Copper Z4 (By similarity)		H		1
METAL		595		595		Copper A1 (By similarity)		H		2
METAL		630		630		Copper A1 (By similarity)		C		2
METAL		630		630		Copper A2 (By similarity)		C		2
METAL		632		632		Copper A2; via carbonyl oxygen (By similarity)		[WH]		2
METAL		634		634		Copper A1 (By similarity)		C		2
METAL		634		634		Copper A2 (By similarity)		C		2
METAL		638		638		Copper A2 (By similarity)		H		2
METAL		641		641		Copper A1 (By similarity)		M		2

Size range:	634-652 amino acids
Related UniRules:	None
Template:	Q51705 (NOSZ_PARDE); P19573 (NOSZ_PSEST): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	in CUPNH, RALSO, RHIME