HAMAP logo

HAMAP rule MF_00716

Send feedback

General rule information [?]

Accession MF_00716
Dates 2-OCT-2003 (Created)
28-NOV-2023 (Last updated, Version 41)
Name NosZ
Scope(s) Bacteria
Template(s) Q51705 (NOSZ_PARDE); P19573 (NOSZ_STUST); [ Recover all ]
Triggered by HAMAP; MF_00716 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NOSZ
Protein name RecName: Full=Nitrous-oxide reductase;
                 EC=1.7.2.4;
AltName: Full=N(2)OR;
AltName: Full=N2O reductase;
                 Flags: Precursor;
Gene name Name=nosZ;

Comments [?]

FUNCTIONNitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide.
CATALYTIC ACTIVITY Reaction=2 Fe(III)-[cytochrome c] + H2O + N2 = 2 Fe(II)-[cytochrome c] + 2 H(+) + nitrous oxide; Xref=Rhea:RHEA:43108, Rhea:RHEA-COMP:10350, Rhea:RHEA-COMP:14399, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17045, ChEBI:CHEBI:17997, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034; EC=1.7.2.4;
COFACTOR Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 2 calcium ions per subunit.;
COFACTOR Name=Cu cation; Xref=ChEBI:CHEBI:23378; Note=Binds 6 Cu cations per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction.;
SUBUNITHomodimer.
SUBCELLULAR LOCATIONPeriplasm.
case <Feature:PS51318>
PTMPredicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
end case
SIMILARITYBelongs to the NosZ family.
SIMILARITYIn the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

Keywords [?]


Gene Ontology [?]

GO:0005509; Molecular function:calcium ion binding
GO:0005507; Molecular function:copper ion binding
GO:0050304; Molecular function:nitrous-oxide reductase activity
GO:0042597; Cellular component:periplasmic space

Cross-references [?]

Pfam PF00394; Cu-oxidase; 1;
NCBIfam TIGR04244; nitrous_NosZ_RR; 1;
NCBIfam TIGR01409; TAT_signal_seq; 1;
PROSITE PS00078; COX2; 1;
PROSITE PS51318; TAT; 0-1;
General Signal; -; 1;

Features [?]

From: NOSZ_PARDE (Q51705)
Key From To Description Tag Condition FTGroup
REGION 554 Cter /note="COX2-like"
BINDING 145 145 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="Z2"
H 1
BINDING 146 146 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="Z3"
H 1
BINDING 194 194 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="Z2"
H 1
BINDING 271 271 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="2"
Y 3
BINDING 274 274 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="2"
E 3
BINDING 282 282 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="2"
M 3
BINDING 288 288 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="2"
D 3
BINDING 335 335 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="2"
[NS] 3
BINDING 337 337 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="Z1"
H 1
BINDING 392 392 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="Z1"
H 1
BINDING 443 443 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="Z3"
H 1
BINDING 464 464 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="1"
K 3
BINDING 479 479 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="1"
E 3
BINDING 504 504 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="Z4"
H 1
BINDING 595 595 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="A1"
H 2
BINDING 630 630 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="A1"
C 2
BINDING 630 630 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="A2"
C 2
BINDING 632 632 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="A2"
[WH] 2
BINDING 634 634 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="A1"
C 2
BINDING 634 634 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="A2"
C 2
BINDING 638 638 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="A2"
H 2
BINDING 641 641 /ligand="Cu cation"
/ligand_id="ChEBI:CHEBI:23378"
/ligand_label="A1"
M 2

Additional information [?]

Size range 634-652 amino acids
Related rules None
Fusion Nter: None Cter: None



View rule in raw text format (no links)