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Annotation rule MF_00772
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General rule information [?]

Accession MF_00772
Dates 16-APR-2004 (Created)
19-JUN-2017 (Last updated, Version 19)
Name OGT
Scope Archaea
Bacteria
Templates O74023 (OGT_THEKO); Q58924 (OGT_METJA); P11742 (OGT_BACSU): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
OGT
Protein name
RecName: Full=Methylated-DNA--protein-cysteine methyltransferase;
EC=2.1.1.63;
AltName: Full=6-O-methylguanine-DNA methyltransferase;
Short=MGMT;
AltName: Full=O-6-methylguanine-DNA-alkyltransferase;
case <OC:Archaea>
Gene name
ogt
end case

Comments [?]

Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Catalytic activity DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.
Subcellular location Cytoplasm.
Miscellaneous This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.
Similarity Belongs to the MGMT family.

Keywords [?]


Gene Ontology [?]

GO:0003908; Molecular function: methylated-DNA-[protein]-cysteine S-methyltransferase activity.
GO:0006307; Biological process: DNA dealkylation involved in DNA repair.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01035; DNA_binding_1; 1;
TIGRFAMs TIGR00589; Ogt; 1;
PROSITE PS00374; MGMT; 1;

Features [?]

From: OGT_THEKO (O74023)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     141     141       Nucleophile; methyl group acceptor     C  

Additional information [?]

Size range 131-220 amino acids
Related rules None
Fusion None