HAMAP rule MF_00772
General rule information
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| Accession | MF_00772 |
| Dates | 16-APR-2004 (Created)
1-JUN-2023 (Last updated, Version 22) |
| Name | OGT |
| Scope(s) |
Archaea Bacteria |
| Template(s) | O74023 (OGT_THEKO); Q58924 (OGT_METJA); P11742 (OGT_BACSU); [ Recover all ] |
| Triggered by |
HAMAP; MF_00772 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | OGT |
| Protein name | RecName: Full=Methylated-DNA--protein-cysteine methyltransferase; EC=2.1.1.63; AltName: Full=6-O-methylguanine-DNA methyltransferase; Short=MGMT; AltName: Full=O-6-methylguanine-DNA-alkyltransferase; |
| case <OC:Archaea> | |
| Gene name | Name=ogt; |
| end case | |
Comments
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| FUNCTION | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| CATALYTIC ACTIVITY | Reaction=a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:24000, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:10132, Rhea:RHEA-COMP:11367, Rhea:RHEA-COMP:11368, ChEBI:CHEBI:29950, ChEBI:CHEBI:82612, ChEBI:CHEBI:85445, ChEBI:CHEBI:85448; EC=2.1.1.63; |
| CATALYTIC ACTIVITY | Reaction=a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:53428, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:10132, Rhea:RHEA-COMP:13555, Rhea:RHEA-COMP:13556, ChEBI:CHEBI:29950, ChEBI:CHEBI:82612, ChEBI:CHEBI:137386, ChEBI:CHEBI:137387; EC=2.1.1.63; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| MISCELLANEOUS | This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles. |
| SIMILARITY | Belongs to the MGMT family. |
Keywords
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Gene Ontology
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| GO:0003908; Molecular function:methylated-DNA-[protein]-cysteine S-methyltransferase activity |
| GO:0006307; Biological process:DNA dealkylation involved in DNA repair |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: OGT_THEKO (O74023) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 141 | 141 | /note="Nucleophile; methyl group acceptor" | C | ||||||||
Additional information
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| Size range | 131-220 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |