HAMAP

Accession	MF_00772
Dates	16-APR-2004 (Created) 11-OCT-2011 (Last updated, Version 15)

Data class

Protein

Predictors

HAMAP; MF_00772; [distribution of match scores in UniProtKB];[seed alignment for MF_00772]

Names

OGT

Identifier

OGT

Protein name

RecName: Full=Methylated-DNA--protein-cysteine methyltransferase; EC=2.1.1.63; AltName: Full=6-O-methylguanine-DNA methyltransferase; Short=MGMT; AltName: Full=O-6-methylguanine-DNA-alkyltransferase;

Gene name

ogt

FUNCTION: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated (By similarity).

CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

MISCELLANEOUS: This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles.

SIMILARITY: Belongs to the MGMT family.

Pfam	PF01035; DNA_binding_1; 1;
TIGRFAMs	TIGR00589; Ogt; 1;
PROSITE	PS00374; MGMT; 1;

Cytoplasm, DNA damage, DNA repair, Transferase, Methyltransferase.

GO:0003908; Molecular function: methylated-DNA-[protein]-cysteine S-methyltransferase activity.
GO:0006307; Biological process: DNA dealkylation involved in DNA repair.
GO:0005737; Cellular component: cytoplasm.

From: OGT_PYRKO (O74023)
Key		From		To		Description		Condition		FTGroup
ACT_SITE		141		141		Nucleophile; methyl group acceptor (By similarity)		C

Size range:	131-176 amino acids
Related UniRules:	None
Template:	O74023 (OGT_PYRKO)
Scope:	Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None