HAMAP rule MF_00782
General rule information
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Accession | MF_00782 |
Dates | 4-MAY-2004 (Created)
1-JUN-2023 (Last updated, Version 26) |
Name | Glut_biosynth |
Scope(s) |
Bacteria Bacillota Pseudomonadota |
Template(s) | Q8DXM9 (GSHAB_STRA5); [ Recover all ] |
Triggered by |
HAMAP; MF_00782 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | GSHAB |
Protein name | RecName: Full=Glutathione biosynthesis bifunctional protein GshAB; AltName: Full=Gamma-GCS-GS; Short=GCS-GS; Includes: RecName: Full=Glutamate--cysteine ligase; EC=6.3.2.2; AltName: Full=Gamma-ECS; Short=GCS; AltName: Full=Gamma-glutamylcysteine synthetase; Includes: RecName: Full=Glutathione synthetase; EC=6.3.2.3; AltName: Full=GSH synthetase; Short=GS; Short=GSH-S; Short=GSHase; AltName: Full=Glutathione synthase; |
Gene name | Name=gshAB; Synonyms=gshF; |
Comments
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FUNCTION | Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine. |
CATALYTIC ACTIVITY | Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L- cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235, ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2; |
CATALYTIC ACTIVITY | Reaction=ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + glutathione + H(+) + phosphate; Xref=Rhea:RHEA:13557, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57305, ChEBI:CHEBI:57925, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.3; |
PATHWAY | Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. |
PATHWAY | Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. |
SUBUNIT | Monomer. |
SIMILARITY | In the N-terminal section; belongs to the glutamate-- cysteine ligase type 1 family. Type 2 subfamily. |
Keywords
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Gene Ontology
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GO:0005524; Molecular function:ATP binding |
GO:0004363; Molecular function:glutathione synthase activity |
GO:0004357; Molecular function:glutamate-cysteine ligase activity |
GO:0006750; Biological process:glutathione biosynthetic process |
Cross-references
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Pfam | PF04262; Glu_cys_ligase; 1; |
NCBIfam | TIGR01435; Glu_cys_lig_rel; 1; |
PROSITE | PS50975; ATP_GRASP; 1; |
Features
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From: GSHAB_STRA5 (Q8DXM9) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | Nter | 333 | /note="Glutamate--cysteine ligase" |
Additional information
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Size range | 750-779 amino acids |
Related rules |
MF_00578 |
Fusion | Nter: None Cter: None |